FASTKD3 siRNA and shRNA Plasmids (m)

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- Target species: mouse; for corresponding human product, see FASTKD3 siRNA and shRNA Plasmids (h)
- Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
- For independent verification of FASTKD3 gene silencing results, individual duplex components or plasmids are also available upon request
- Get details about si/shRNA Gene Silencers
- Gene silencers are intended for noncommercial and research use only. Click here for details.

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Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Mouse	Fastkd3	69577	13 B3	XM_006517364, XM_006517365, XM_006517366, XM_006517367, XM_017315595, NM_001330423, NM_027123	Q8BSN9	N/A

Description

10 µM, 50-100 transfections
siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
for independent verification of FASTKD3 gene silencing results, individual siRNA duplex components are also available upon request
contact Technical Service for availability of control antibody
sqRT-PCR Primer also available: FASTKD3 (m)-PR: sc-145077-PR, 20 µl
Get details about si/shRNA Gene Silencers

See Protocol Online.

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Description

20 µl of each primer set is provided at a concentration of 10 µM
FASTKD3 Primer (m) includes A set (forward & reverse) and B set (forward & reverse) primers
Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Description

20 µg, up to 20 transfections
shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to FASTKD3 siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
shRNA Plasmids are intended only for non-commercial use.
contact Technical Service for availability of control antibody
sqRT-PCR Primer also available: FASTKD3 (m)-PR: sc-145077-PR, 20 µl

See Protocol Online.

DOWNLOADS

QUICK LINKS

Description

200 µl viral stock containing 1x 10⁶ infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to FASTKD3 siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
Lentiviral Particles are intended for non-commercial use.

See Protocol Online.

DOWNLOADS

QUICK LINKS