Fast Red ITR Salt (CAS 27580-14-9)

Fast Red ITR Salt, also known as 1-(2,4-Dinitrophenylazo)-2-naphthol, is a synthetic organic compound used as a dye and staining reagent in various biological and histological applications. It belongs to the azo dye family and is characterized by its bright red color. Fast Red ITR Salt has excellent solubility in water, making it easily dispersible and suitable for use in aqueous-based systems. Its vibrant red hue allows for clear visualization and differentiation of stained samples, aiding in the examination of cellular structures and tissue components. In the field of histology and pathology, Fast Red ITR Salt is frequently utilized as a chromogen in immunohistochemistry (IHC) and immunocytochemistry (ICC) procedures. It serves as a substrate for the detection of specific target antigens, usually in conjunction with an appropriate antibody conjugated with an enzyme such as alkaline phosphatase or horseradish peroxidase. The resulting colored reaction product provides visual evidence of the presence and localization of the target molecule within the sample. Fast Red ITR Salt is also employed in other biological techniques, including fluorescence microscopy and cell labeling studies. It can be used to label cells or specific cellular components, allowing researchers to track and identify particular structures of interest. In summary, Fast Red ITR Salt is a versatile red dye widely used in biological and histological applications. Its solubility in water, bright color, and compatibility with various staining techniques make it useful for visualizing and studying cellular structures and molecular targets.