FARSLB Antibody (J-02): sc-100985

FARSLB Antibody (J-02) is a mouse monoclonal IgG2a kappa light chain antibody that detects FARSLB protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-FARSLB antibody (J-02) is available as the non-conjugated format. FARSLB, also known as Phenylalanine--tRNA ligase beta chain, FARSB, FRSB, or PheHB, is a crucial 589 amino acid cytoplasmic protein that serves as the regulatory beta subunit of phenylalanine-tRNA synthetase. FARSLB plays a vital role in protein synthesis by catalyzing the attachment of L-phenylalanine to corresponding tRNA, a process essential for accurate translation of genetic information into functional proteins. The enzymatic activity of FARSLB is ATP-dependent, resulting in the formation of an L-phenylalanine-tagged tRNA and release of AMP and diphosphate. FARSLB is predominantly expressed in various tissues, including brain, heart, kidney, pancreas, placenta, and skeletal muscle, with expression levels notably elevated in malignant cell lines, indicating potential involvement in carcinogenesis. FARSLB′s interaction with specific proteins involved in the tRNA synthetase complex further underscores importance in cellular function and regulation, making FARSLB monoclonal antibody (J-02) an invaluable tool for researchers studying protein synthesis and disease implications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FARSLB Antibody (J-02) References:

1. Human phenylalanyl-tRNA synthetase: cloning, characterization of the deduced amino acid sequences in terms of the structural domains and coordinately regulated expression of the alpha and beta subunits in chronic myeloid leukemia cells.  |  Rodova, M., et al. 1999. Biochem Biophys Res Commun. 255: 765-73. PMID: 10049785
2. Cloning of the cDNA encoding phenylalanyl tRNA synthetase regulatory alpha-subunit-like protein whose expression is down-regulated during differentiation.  |  Zhou, X., et al. 1999. Gene. 233: 13-9. PMID: 10375616
3. Cloning and expression of human phenylalanyl-tRNA synthetase in Escherichia coli: comparative study of purified recombinant enzymes.  |  Moor, N., et al. 2002. Protein Expr Purif. 24: 260-7. PMID: 11858721
4. Prokaryotic and eukaryotic tetrameric phenylalanyl-tRNA synthetases display conservation of the binding mode of the tRNA(Phe) CCA end.  |  Moor, N., et al. 2003. Biochemistry. 42: 10697-708. PMID: 12962494
5. A series of spirocyclic analogues as potent inhibitors of bacterial phenylalanyl-tRNA synthetases.  |  Yu, XY., et al. 2004. Bioorg Med Chem Lett. 14: 1339-42. PMID: 14980694
6. Role of low-molecular-weight substrates in functional binding of the tRNAPhe acceptor end by phenylalanyl-tRNA synthetase.  |  Vasil'eva, IA., et al. 2004. Biochemistry (Mosc). 69: 143-53. PMID: 15000680
7. Expression of a gene encoding a tRNA synthetase-like protein is enhanced in tumorigenic human myeloid leukemia cells and is cell cycle stage- and differentiation-dependent.  |  Sen, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 6164-9. PMID: 9177188

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FARSLB Antibody (J-02) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-FARSLB Antibody (J-02) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FARSLB Antibody (J-02) Specifications

Alternate Product Name: FARSLB

Product Clone Name: J-02

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 234-342

Epitope Target: FARSLB

Epitope Species: human

Product Reactivity/Detection: FARSLB

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for FARSLB Antibody (J-02): N/A