FAP Antibody (F11-24): sc-65398

FAP Antibody (F11-24) is a mouse monoclonal IgG antibody that detects FAP protein of human origin by immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-FAP antibody (F11-24) is available as a non-conjugated format. Fibroblast activation protein (FAP) is a crucial cell surface glycoprotein and serine protease predominantly expressed in fetal mesenchymal tissues and in fibroblasts of epithelial cancers. FAP (F11-24) antibody promotes cellular proliferation, contributing to tumor growth and progression. During embryonic development, FAP is essential for remodeling developing tissues, highlighting FAP′s importance in both normal and pathological processes. Structurally, FAP is an integral membrane gelatinase composed of N-glycosylated, proteolytically inactive subunits, which underscores unique functional properties. FAP expression on chondrocyte membranes is upregulated by cytokines interleukin-1 and oncostatin M, and increases in patients with osteoarthritis. This expression is co-localized with matrix metalloproteinases MMP-1 and MMP-13, as well as CD44 variants v3 and v7/8, indicating involvement in complex cellular interactions. Mice lacking all copies of FAP gene remain fertile but exhibit developmental defects and altered cancer susceptibility, further emphasizing FAP′s critical role in both development and disease.

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FAP Antibody (F11-24) References:

1. Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein alpha.  |  Aertgeerts, K., et al. 2005. J Biol Chem. 280: 19441-4. PMID: 15809306
2. Fibroblast activation protein-alpha and dipeptidyl peptidase IV (CD26): cell-surface proteases that activate cell signaling and are potential targets for cancer therapy.  |  Kelly, T. 2005. Drug Resist Updat. 8: 51-8. PMID: 15939342
3. Characterization of cancer stroma markers: in silico analysis of an mRNA expression database for fibroblast activation protein and endosialin.  |  Dolznig, H., et al. 2005. Cancer Immun. 5: 10. PMID: 16076089
4. 'Increased expression of seprase, a membrane-type serine protease, is associated with lymph node metastasis in human colorectal cancer'.  |  Iwasa, S., et al. 2005. Cancer Lett. 227: 229-36. PMID: 16196122
5. Development of Quinoline-Based Theranostic Ligands for the Targeting of Fibroblast Activation Protein.  |  Lindner, T., et al. 2018. J Nucl Med. 59: 1415-1422. PMID: 29626119
6. Fibroblast Activation Protein (FAP)-Targeted CAR-T Cells: Launching an Attack on Tumor Stroma.  |  Bughda, R., et al. 2021. Immunotargets Ther. 10: 313-323. PMID: 34386436
7. Comprehensive Analysis of Fibroblast Activation Protein Expression in Interstitial Lung Diseases.  |  Yang, P., et al. 2023. Am J Respir Crit Care Med. 207: 160-172. PMID: 35984444
8. Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.  |  Yang, AT., et al. 2023. Cell Mol Gastroenterol Hepatol. 15: 841-867. PMID: 36521660
9. The gene for fibroblast activation protein alpha (FAP), a putative cell surface-bound serine protease expressed in cancer stroma and wound healing, maps to chromosome band 2q23.  |  Mathew, S., et al. 1995. Genomics. 25: 335-7. PMID: 7774951
10. Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers.  |  Scanlan, MJ., et al. 1994. Proc Natl Acad Sci U S A. 91: 5657-61. PMID: 7911242
11. Identification of the 170-kDa melanoma membrane-bound gelatinase (seprase) as a serine integral membrane protease.  |  Piñeiro-Sánchez, ML., et al. 1997. J Biol Chem. 272: 7595-601. PMID: 9065413
12. Molecular cloning of seprase: a serine integral membrane protease from human melanoma.  |  Goldstein, LA., et al. 1997. Biochim Biophys Acta. 1361: 11-9. PMID: 9247085

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FAP Antibody (F11-24) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-FAP Antibody (F11-24) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FAP Antibody (F11-24) Specifications

Alternate Product Name: FAP

Product Clone Name: F11-24

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: FAP

Epitope Species: human

Product Reactivity/Detection: FAP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, FCM

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for FAP Antibody (F11-24): N/A