FAM171A1 Double Nickase Plasmid (h): sc-415117-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM171A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FAM171A1 Double Nickase Plasmid (h) and FAM171A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FAM171A1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM171A1 Double Nickase Plasmid (h) | sc-415117-NIC | 20 µg | $410.00 | |||
FAM171A1 Double Nickase Plasmid (h2) | sc-415117-NIC-2 | 20 µg | $410.00 |
FAM171A1 encodes a transmembrane protein implicated in regulation of cell–cell interactions and membrane-associated signaling, with enriched expression in neural tissues. Emerging evidence links FAM171A1 to axon guidance and neurite outgrowth programs, suggesting a role in shaping neuronal connectivity and synaptic organization. As a putative modulator of surface receptor context and adhesion-dependent signaling, FAM171A1 may influence cytoskeletal dynamics and developmental patterning pathways. Altered expression or genetic variation in FAM171A1 has been explored in studies of neurodevelopmental and neuropsychiatric phenotypes, supporting its relevance for mechanistic investigations in nervous system biology.
FAM171A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FAM171A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FAM171A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FAM171A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FAM171A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.