F4/80 Antibody (6A545): sc-71085
- F4/80 Antibody (6A545) is a rat monoclonal IgG2a, cited in 16 publications, provided at 100 µg/ml
- raised against cultured bone marrow-derived macrophages of mouse origin
- recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB, IF, IHC(P) and FCM
- See F4/80 (C-7): sc-377009 for F4/80 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for F4/80 Antibody (6A545). This work is in progress.
F4/80 Antibody (6A545) is a rat monoclonal IgG2a antibody that detects F4/80 in mouse, rat, and human samples through applications such as western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). F4/80, a member of the epidermal growth factor (EGF)-TM7 family, is predominantly expressed on macrophages and serves as a critical marker for identifying mature macrophage populations in various tissues, including Kupffer cells in the liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 is encoded by a gene cluster located on human chromosome 19p13 and features an extended extracellular region with multiple N-terminal EGF domains, which is essential for protein interaction and signaling pathways. F4/80 undergoes extensive N-linked glycosylation and some O-linked glycosylation, modifications that are crucial for stability, localization, and function. While the precise role of F4/80 in macrophage biology remains to be fully elucidated, F4/80 is believed to play a significant part in macrophage adhesion, migration, and possibly in G-protein coupled signaling mechanisms, highlighting its importance in immune responses and tissue homeostasis. F4/80 (6A545) antibody is an invaluable tool for researchers studying macrophage biology and related immunological processes.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
F4/80 Antibody (6A545) | sc-71085 | 100 µg/ml | $322.00 |