Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EXT1 Double Nickase Plasmid (h): sc-404635-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EXT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EXT1 Double Nickase Plasmid (h) and EXT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EXT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EXT1 Antibody (A-7): sc-515144
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EXT1 Double Nickase Plasmid (h) | sc-404635-NIC | 20 µg | $410.00 | |||
EXT1 Double Nickase Plasmid (h2) | sc-404635-NIC-2 | 20 µg | $410.00 |
EXT1 encodes exostosin-1, a Golgi-resident glycosyltransferase that forms a hetero-oligomeric complex with EXT2 to catalyze polymerization of heparan sulfate chains on proteoglycan core proteins. By controlling heparan sulfate biosynthesis, EXT1 shapes extracellular matrix organization and modulates the distribution and signaling of heparin-binding ligands, influencing pathways such as FGF, WNT, Hedgehog, and BMP. Altered EXT1 function perturbs proteoglycan sulfation patterns and ligand–receptor interactions that regulate development, morphogenesis, and cell–matrix communication. Germline and somatic disruptions in EXT1 are associated with abnormal skeletal growth and tumor-related phenotypes, making it a useful node for studying glycobiology and signaling-dependent cellular behaviors.
EXT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EXT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EXT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EXT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EXT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.