EX 527 CAS: 49843-98-3
MF: C13H13ClN2O
MW: 248.71
A selective inhibitor of SIRT1 over SIRT2 and SIRT3.

EX 527 (CAS 49843-98-3)

EX 527 | CAS 49843-98-3 is rated 5.0 out of 5 by 1.
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Synonym: SIRT1 Inhibitor III; Selisistat
Application: A selective inhibitor of SIRT1 over SIRT2 and SIRT3
CAS Number: 49843-98-3
Purity: ≥95%
Molecular Weight: 248.71
Molecular Formula: C13H13ClN2O
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

EX 527 is a potent and selective inhibitor of the SIRT1 class III histone deacetylase enzyme, thought to block the release of deacetylated peptide and O-acetyl-ADP-ribose from the enzyme following the deacetylation process. EX 527 has been used a powerful tool for studying the relationship between SIRT1 and cell regulation. The deacetylation of cortactin, a protein responsible for rearrangements of the actin cytoskeleton, is associated with cell motility and possibly tumorigenesis, and blocking of this deacetylation by EX 527 correlated to a decrease in cell motility. Blocking of SIRT1 by EX 527 also demonstrated that deacetylation of the important tumor suppressor protein p53 is mediated by SIRT1 as well. EX 527 inhibits other sirtuin family deacetylases only at much higher concentrations (IC50 = 19.6 and 48.7 μM for SIRT2 and SIRT3, respectively).


1. Napper, Andrew D., et al., 2005. Discovery of indoles as potent and selective inhibitors of the deacetylase SIRT1. Journal of medicinal chemistry. 48(25): 8045-54. PMID: 16335928
2. Solomon, Jonathan M., et al., 2006. Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage. Molecular and cellular biology. 26(1): 28-38. PMID: 16354677
3. Milne, Jill C., et al., 2007. Small molecule activators of SIRT1 as therapeutics for the treatment of type 2 diabetes. Nature. 450(7170): 712-6. PMID: 18046409
4. Zhang, Y., et al., 2009. Deacetylation of cortactin by SIRT1 promotes cell migration. Oncogene. 28(3): 445-60. PMID: 18850005

Physical State :
Solubility :
Soluble in DMSO (10 mg/ml), ethanol (5 mg/ml), DMF (~20 mg/ml), and DMSO:PBS (1:1 pH 7.2) (~0.5 mg/ml).
Storage :
Store at 4° C
Melting Point :
178° C
Boiling Point :
531.72° C at 760 mmHg (Predicted)
Density :
1.39 g/cm3 (Predicted)
Refractive Index :
n20D 1.69 (Predicted)
IC50 :
SIRT1: IC50 = 98 nM; SIRT2: IC50 = 19.6 µM; SIRT3: IC50 = 48.7 µM
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
MDL Number :

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EX 527  Product Citations

See how others have used EX 527. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 14 total

PMID: # 30917412  Igarashi, M.|Miura, M.|Williams, E.|Jaksch, F.|Kadowaki, T.|Yamauchi, T.|Guarente, L.| et al. 2019. Aging Cell. 18: e12935.

PMID: # 30213795  Funato, K.|Hayashi, T.|Echizen, K.|Negishi, L.|Shimizu, N.|Koyama-Nasu, R.|Nasu-Nishimura, Y.|Morishita, Y.|Tabar, V.|Todo, T.|Ino, Y.|Mukasa, A.|Saito, N.|Akiyama, T.| et al. 2018. EMBO Rep. 19:

PMID: # 28978059  Bhanot, H. et al. 2017. Oncotarget. 8: 67639-67650.

PMID: # 26891914  Graham, RM. et al. 2016. Experimental & molecular medicine. 48: e210.

PMID: # 26052531  Wielgosz, MM. et al. 2015. Molecular therapy. Methods & clinical development. 2: 14063.

PMID: # 25401748  Yang, Y. et al. 2015. Journal of pineal research. 58: 61-70.

PMID: # 25710021  He, W. et al. 2015. BioMed research international. 2015: 684242.

PMID: # 26307266  Guida, N. et al. 2015. Toxicology and applied pharmacology. 288: 387-98.

PMID: # 24982422  Rodriguez, M. et al. 2014. J Biol Chem. 289: 22942-22957.

PMID: # 24990896  Quadri, S. et al. 2014. American journal of physiology. Renal physiology. 307: F593-600.

Citations 1 to 10 of 14 total

What are the storage conditions necessary for this product after it has been dissolved in DMSO? How long can it be stored like this?

Asked by: two2igm05
Thank you for your question. EX 527, sc-203044, can be stored for 3 years at -20℃ as a powder or for 6 months at -80℃ in solvent.
Answered by: TechService7
Date published: 2018-10-10
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Rated 5 out of 5 by from Atkins Atkins, KM. et al. (PubMed 25159152) found that EX 527, a selective inhibitor of SIRT1 deacetylation activity, restored p53 acetylation and p21 induction, as well as p21-dependent cell cycle arrest in PACS-2 knockdown cells. -SCBT Publication Review
Date published: 2015-01-02
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