ETFB Antibody (F-1): sc-514807

ETFB Antibody (F-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects ETFB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ETFB antibody (F-1) is available as the non-conjugated form. ETFB protein, also known as electron transfer flavoprotein subunit beta, FP585, MADD, or beta-ETF, is a crucial 255 amino acid protein that plays a significant role in cellular metabolism. ETFB (F-1) antibody targets a protein primarily located in the mitochondrion matrix, where ETFB is abundantly expressed in liver, heart, and skeletal muscle, with lower expression levels in brain, placenta, lung, kidney, and pancreas. ETFB′s mitochondrial localization is important for its function as an electron shuttle, facilitating transfer of electrons between primary flavoprotein dehydrogenases and membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase, which is essential for efficient energy production. ETFB protein exists as a heterodimer composed of alpha and beta subunits, utilizing flavin adenine dinucleotide (FAD) as a cofactor and binding one adenosine monophosphate (AMP) per subunit. ETFB gene is located on human chromosome 19q13.33 and mouse chromosome 7 B4, and mutations in this gene have been associated with glutaric aciduria type 2B (GA2B), an autosomal recessive disorder affecting fatty acid, amino acid, and choline metabolism.

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ETFB Antibody (F-1) References:

1. A polymorphic variant in the human electron transfer flavoprotein alpha-chain (alpha-T171) displays decreased thermal stability and is overrepresented in very-long-chain acyl-CoA dehydrogenase-deficient patients with mild childhood presentation.  |  Bross, P., et al. 1999. Mol Genet Metab. 67: 138-47. PMID: 10356313
2. Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple acyl-CoA dehydrogenation deficiency.  |  Olsen, RK., et al. 2003. Hum Mutat. 22: 12-23. PMID: 12815589
3. RsaI RFLP for electron transport flavoprotein-beta(ETFB).  |  Royal, V., et al. 1991. Nucleic Acids Res. 19: 4021. PMID: 1677763
4. ATP5B and ETFB metabolic markers in children with congenital hydronephrosis.  |  Zhao, Q., et al. 2016. Mol Med Rep. 14: 5111-5115. PMID: 27840937
5. Integrated analysis of ultra-deep proteomes in cortex, cerebrospinal fluid and serum reveals a mitochondrial signature in Alzheimer's disease.  |  Wang, H., et al. 2020. Mol Neurodegener. 15: 43. PMID: 32711556
6. A 20-year Clinical and Genetic Neuromuscular Cohort Analysis in Lebanon: An International Effort.  |  Megarbane, A., et al. 2022. J Neuromuscul Dis. 9: 193-210. PMID: 34602496
7. Mutations and polymorphisms of the gene encoding the beta-subunit of the electron transfer flavoprotein in three patients with glutaric acidemia type II.  |  Colombo, I., et al. 1994. Hum Mol Genet. 3: 429-35. PMID: 7912128
8. Assignment of the gene encoding the beta-subunit of the electron-transfer flavoprotein (ETFB) to human chromosome 19q13.3.  |  Antonacci, R., et al. 1994. Genomics. 19: 177-9. PMID: 8188225
9. cDNA cloning and mitochondrial import of the beta-subunit of the human electron-transfer flavoprotein.  |  Finocchiaro, G., et al. 1993. Eur J Biochem. 213: 1003-8. PMID: 8504797
10. Assignment of Etfdh, Etfb, and Etfa to chromosomes 3, 7, and 13: the mouse homologs of genes responsible for glutaric acidemia type II in human.  |  White, RA., et al. 1996. Genomics. 33: 131-4. PMID: 8617498
11. Three-dimensional structure of human electron transfer flavoprotein to 2.1-A resolution.  |  Roberts, DL., et al. 1996. Proc Natl Acad Sci U S A. 93: 14355-60. PMID: 8962055
12. Fatty acid oxidation disorders as primary cause of sudden and unexpected death in infants and young children: an investigation performed on cultured fibroblasts from 79 children who died aged between 0-4 years.  |  Lundemose, JB., et al. 1997. Mol Pathol. 50: 212-7. PMID: 9350306

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ETFB Antibody (F-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ETFB Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ETFB Antibody (F-1) Specifications

Alternate Product Name: ETFB

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 180-201

Epitope Target: ETFB

Epitope Species: human

Product Reactivity/Detection: ETFB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ETFB Antibody (F-1): P: Blocking Peptide