Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Estrogen Receptor beta Antibody (B-1): sc-390243. Western blot analysis of ERβ expression in I-11.15 whole cell lysate.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Estrogen Receptor beta Lentiviral Activation Particles (m): sc-420232-LAC
- Target species: mouse
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- Estrogen Receptor beta Lentiviral Activation Particles (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- Estrogen Receptor beta Lentiviral Activation Particles (m) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Estrogen Receptor beta Lentiviral Activation Plasmid (m) and Estrogen Receptor beta Lentiviral Activation Plasmid (m2) target distinct regulatory regions of the Esr2 promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: Estrogen Receptor beta Antibody (B-1): sc-390243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Estrogen Receptor beta Lentiviral Activation Particles (m) | sc-420232-LAC | 200 µl | $455.00 |