Estrogen Receptor beta Double Nickase Plasmid (m): sc-420232-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Estrogen Receptor beta Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Estrogen Receptor beta Double Nickase Plasmid (m) and Estrogen Receptor beta Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Esr2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Estrogen Receptor beta Antibody (B-1): sc-390243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Estrogen Receptor beta Double Nickase Plasmid (m) | sc-420232-NIC | 20 µg | $410.00 | |||
Estrogen Receptor beta Double Nickase Plasmid (m2) | sc-420232-NIC-2 | 20 µg | $410.00 |
Mouse Esr2 encodes estrogen receptor beta (ERβ), a ligand-activated nuclear receptor that functions as a transcription factor regulating genes involved in reproductive biology, neural signaling, immune modulation, and tissue homeostasis. Upon estrogen binding, ERβ engages estrogen response elements and coregulator complexes to shape chromatin state and transcription within steroid hormone signaling networks, with extensive crosstalk to MAPK/ERK and PI3K/AKT pathways. ERβ activity influences cell-cycle control, differentiation programs, mitochondrial function, and inflammatory gene expression in a context-dependent manner. Dysregulated Esr2 signaling has been linked to altered fertility phenotypes, neurobehavioral changes, and hormone-responsive pathophysiology relevant to metabolic and inflammatory disorders in mouse models.
Estrogen Receptor beta Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Esr2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Esr2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Esr2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Esr2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.