Estrogen Receptor alpha Double Nickase Plasmid (h): sc-400011-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Estrogen Receptor alpha Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Estrogen Receptor alpha Double Nickase Plasmid (h) and Estrogen Receptor alpha Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ESR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Estrogen Receptor alpha Antibody (F-10): sc-8002
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Estrogen Receptor alpha Double Nickase Plasmid (h) | sc-400011-NIC | 20 µg | $410.00 | |||
Estrogen Receptor alpha Double Nickase Plasmid (h2) | sc-400011-NIC-2 | 20 µg | $410.00 |
ESR1 encodes estrogen receptor alpha (ERα), a ligand-activated nuclear receptor that functions as a transcription factor coordinating estrogen-responsive gene programs. Upon hormone binding, ERα engages estrogen response elements and coregulator complexes to modulate chromatin accessibility and transcription, integrating signaling across PI3K/AKT, MAPK/ERK, and cell-cycle regulatory networks. ERα activity shapes cellular proliferation, differentiation, and metabolic homeostasis in multiple tissues, and altered ESR1 regulation or variant forms are frequently studied in hormone-responsive tumor biology and endocrine signaling dysregulation. Because ERα-dependent transcription intersects with epigenetic control and receptor crosstalk, ESR1 is a central target for dissecting steroid receptor signaling and transcriptional network architecture.
Estrogen Receptor alpha Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ESR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ESR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ESR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ESR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.