ERR beta / gamma Antibody (E-1) recommended for detection of ERRβ and ERRγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

ERRβ/γ Antibody (E-1): sc-376449

ERRβ/γ Antibody (E-1) is rated 3.8 out of 5 by 5.
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Datasheets

    • Anti-ERRβ/γ Antibody (E-1) is a mouse monoclonal IgG1 (kappa light chain) ERRβ/γ antibody provided at 200 µg/ml
    • raised against amino acids 251-316 mapping within an internal region of ERRβ of human origin
    • Anti-ERR beta / gamma Antibody (E-1) is recommended for detection of ERRβ and ERRγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ERR beta / gamma Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-376449 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERRβ/γ (E-1): sc-376449.
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Cannot make the antibody (sc-376449) work on PFA-fixed and frozen sections even in a dilution 1:50 and with recommended secondary antibodies (sc-516140, sc-516141).

Asked by: ElenaS
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-09-11

Can ERRβ/γ (E-1): sc-376449 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, ERRβ/γ (E-1): sc-376449 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Not for IF Tested IF on frozen sections of 4% PFA fixed mouse cerebellum, no specific signal was detected.
Date published: 2018-07-30
Rated 5 out of 5 by from Works great for mouse tissue IF Great signal in PFA-fixed, frozen mouse tissue w/little to no background.
Date published: 2018-04-03
Rated 3 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-06-04
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear and Produced great immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-02-17
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