ERI-1 Antibody (B-10): sc-137089

ERI-1 Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects ERI-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ERI-1 (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERI-1, also known as Protein 3′hExo, plays a crucial role in RNA interference regulation by degrading histone mRNA after replication, which is essential for maintaining proper gene expression levels. ERI-1 exhibits a high affinity for the stem-loop structure of replication-dependent histone pre-mRNAs, requiring the specific 5′-ACCCA-3′ sequence found within this structure. The interaction between ERI-1 and a stem-loop binding protein (SLBP) is vital, as they target opposite faces of a highly conserved stem-loop RNA scaffold, facilitating the degradation process at the 3′ end of histone mRNA. Understanding ERI-1′s function in RNA regulation provides insights into gene expression control mechanisms and their potential implications for various biological processes and diseases.

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ERI-1 Antibody (B-10) References:

1. A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans.  |  Kennedy, S., et al. 2004. Nature. 427: 645-9. PMID: 14961122
2. Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER.  |  Sobering, AK., et al. 2004. Cell. 117: 637-48. PMID: 15163411
3. Endogenous inhibitors of RNA interference in Caenorhabditis elegans.  |  Timmons, L. 2004. Bioessays. 26: 715-8. PMID: 15221853
4. High doses of siRNAs induce eri-1 and adar-1 gene expression and reduce the efficiency of RNA interference in the mouse.  |  Hong, J., et al. 2005. Biochem J. 390: 675-9. PMID: 16004606
5. Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants.  |  Wang, D., et al. 2005. Nature. 436: 593-7. PMID: 16049496
6. RNA interference is an antiviral defence mechanism in Caenorhabditis elegans.  |  Wilkins, C., et al. 2005. Nature. 436: 1044-7. PMID: 16107852
7. Dampening the silencing effect of RNA interference in mammals.  |  Zhang, J. 2005. Biochem J. 390: e5-6. PMID: 16145728
8. Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways.  |  Duchaine, TF., et al. 2006. Cell. 124: 343-54. PMID: 16439208
9. Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans.  |  Lee, RC., et al. 2006. RNA. 12: 589-97. PMID: 16489184

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERI-1 Antibody (B-10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ERI-1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERI-1 Antibody (B-10) Specifications

Alternate Product Name: ERI-1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 161-295

Epitope Target: ERI-1

Epitope Species: human

Product Reactivity/Detection: ERI-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ERI-1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent