eRF3b Antibody (32): sc-135899

eRF3b Antibody (32) is a mouse monoclonal IgG1 antibody that detects eRF3b of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-eRF3b antibody (32) is available as a non-conjugated format. eRF3b, also known as Gspt2 (G1 to S phase transition 2), is a crucial 632 amino acid cytoplasmic protein that plays a significant role in translation termination, specifically in recognizing the termination codons UAA, UAG, and UGA. This function is vital for ensuring accurate completion of protein synthesis, which is essential for cellular function and homeostasis. eRF3b is highly expressed in the brain, where eRF3b is involved in protein synthesis regulation, and exhibits GTPase activity dependent on both the ribosome and eRF1, indicating importance in the translation process. eRF3b is moderately expressed in the spleen and lung, with weaker expression levels in the heart, liver, and kidney, and contains three GTP binding sites, making eRF3b a key component of the mRNA surveillance SURF complex. eRF3b′s ability to stimulate the release factor activity of eRF1 further underscores its critical role in cellular processes, including cell cycle progression.

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eRF3b Antibody (32) References:

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2. Mouse GSPT2, but not GSPT1, can substitute for yeast eRF3 in vivo.  |  Le Goff, C., et al. 2002. Genes Cells. 7: 1043-57. PMID: 12354098
3. Involvement of human release factors eRF3a and eRF3b in translation termination and regulation of the termination complex formation.  |  Chauvin, C., et al. 2005. Mol Cell Biol. 25: 5801-11. PMID: 15987998
4. Evolution of translation termination factor eRF3: is GSPT2 generated by retrotransposition of GSPT1's mRNA?  |  Zhouravleva, G., et al. 2006. IUBMB Life. 58: 199-202. PMID: 16754297
5. [Conservation of the MC domains in eukaryotic termination factor eRF3].  |  Zhuravleva, GA., et al. 2007. Genetika. 43: 38-44. PMID: 17333937
6. Human eukaryotic release factor 3a depletion causes cell cycle arrest at G1 phase through inhibition of the mTOR pathway.  |  Chauvin, C., et al. 2007. Mol Cell Biol. 27: 5619-29. PMID: 17562865
7. [Evaluation of the gene encoding translation termination factor eRF3 as a possible phylogenetic marker].  |  Tarasov, OV., et al. 2008. Mol Biol (Mosk). 42: 937-46. PMID: 19140313
8. Characterization of a high copy number amplification at 6q24 in pancreatic cancer identifies c-myb as a candidate oncogene.  |  Wallrapp, C., et al. 1997. Cancer Res. 57: 3135-9. PMID: 9242439
9. Molecular cloning of a novel member of the eukaryotic polypeptide chain-releasing factors (eRF). Its identification as eRF3 interacting with eRF1.  |  Hoshino, S., et al. 1998. J Biol Chem. 273: 22254-9. PMID: 9712840