Date published: 2025-11-5
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western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721
Cet anticorps polyclonal n'est plus commercialisé. Voir l'anticorps monoclonal Estrogen Receptor alpha (F-10) que nous recommandons (données d'échantillonnage indiquées).
western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721
Cet anticorps polyclonal n'est plus commercialisé. Voir l'anticorps monoclonal Estrogen Receptor alpha (F-10) que nous recommandons (données d'échantillonnage indiquées).
Cet anticorps polyclonal n'est plus commercialisé. Voir l'anticorps monoclonal Estrogen Receptor alpha (F-10) que nous recommandons (données d'échantillonnage indiquées).

Anticorps ERα (MC-20): sc-542

4.8(20)
Écrire une critiquePoser une question

  • L'anticorps ER alpha MC-20 est un polyclonal IgG de lapin fourni en 100 µg/ml
  • épitope situé au niveau C-terminus de ERα d'origine mouse
  • Anticorps polyclonal arrêté

VOIR ÉGALEMENT...

Anticorps supplémentaires Estrogen Receptor incluant Estrogen Receptor alpha, ER β, ERR α, ERR β et ERR γ

Informations pour la commande

Estrogen Receptor alpha (F-10): sc-8002 [ Monoclonal recommandé en remplacement de ERα (MC-20) ]

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Estrogen Receptor alpha (F-10)

sc-8002
200 µg/ml
$316.00

Estrogen Receptor alpha (F-10): m-IgG Fc BP-HRP Kit

sc-528195
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha (F-10): m-IgG2a BP-HRP Kit

sc-547044
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha (F-10): m-IgGκ BP-HRP Kit

sc-520517
200 µg Ab, 40 µg BP
$354.00

Anticorps Estrogen Receptor alpha (F-10) X

sc-8002 X
200 µg/0.1 ml
$316.00

Anticorps Estrogen Receptor alpha (F-10) AC

sc-8002 AC
500 µg/ml, 25% agarose
$416.00

Anticorps Estrogen Receptor alpha (F-10) Alexa Fluor® 546

sc-8002 AF546
200 µg/ml
$357.00

Anticorps Estrogen Receptor alpha (F-10) Alexa Fluor® 594

sc-8002 AF594
200 µg/ml
$357.00

Anticorps Estrogen Receptor alpha (F-10) Alexa Fluor® 680

sc-8002 AF680
200 µg/ml
$357.00

Anticorps Estrogen Receptor alpha (F-10) Alexa Fluor® 790

sc-8002 AF790
200 µg/ml
$357.00

Estrogen Receptor alpha (F-10) peptide neutralisant

sc-8002 P
100 µg/0.5 ml
$68.00

sc-8002 is not in the mouse tissues, I tried so hard with different antigen retrieval conditions, for both paraffin and cryosections on the mouse mammary gland. Really headache that SC542 is not available anymore.

Posée par: slimpopo
Thank you for your question. The ERα Antibody (F-10): sc-8002 is specific for an epitope mapping between amino acids 576-595 at the C-terminus of ERα of human origin and it is recommended for detection of ERα of human origin by WB, IP, IF, IHC(P) and ELISA. We do not recommend this antibody for detection of its epitope in murine samples. You can find alternative antibodies specific against the murine epitope by following this link: https://www.scbt.com/scbt/browse/er-alpha-Antibodies/_/N-1qbf0ke. For further information please contact our technical service directly.
Répondue par: SCBT Heidelberg Support
Date de publication: 2019-01-24

Hi Santa Cruz Biotechnology representative, I have an ERalpha (D-4) sc-514910 that I bought in 2016. I don't see it listed on the website and I was wondering if there is a reason for this.

Posée par: NotTom
Thank you for your inquiry. sc-514910 is polyclonal antibody and all the polyclonal antibodies have been discontinued since 2016. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Répondue par: Tech Service
Date de publication: 2018-08-14

Dear Santa Cruz, Why was the very good antibody against the ER-alpha receptor SC-542 discontinued ?? Look at the reference list how many studies were published with this antibody. I also worked with the antibody (cf. Kruijver et al., 2002 JCN). Sincerely

Posée par: Kruijver
Thank you for your question. All our polyclonal antibodies have been discontinued, we are now monoclonal specific. We offer many excellent ER-alpha specific monoclonal antibodies that are fine alternatives to sc-542, such as sc-8002, sc-787 and sc-8005. You can find them here: https://www.scbt.com/scbt/browse/estrogen-receptor-Antibodies/_/N-cuom06
Répondue par: Tech Service
Date de publication: 2017-11-21

Hi, I am wondering if you guys still have the old stock of sc-542 antibody that used to be 200ug/ml? I find that that one works better for my experiments. Please let me know whether I can purchase the old stock. Thank you! Yichen

Posée par: Yichen
Thank you for your question. We have completely switched over all of our stock of this rabbit polyclonal, ERalpha Antibody (MC-20): sc-542 to the new concentration of 100 µg/ml. The difference in antibody performance you are experiencing may be more related to the lot number of the product, rather than the concentration. This is a rabbit polyclonal antibody and new lots are purified from different host animals. As a result, it is typical to observe variation between different lots. And each new lot must be optimized. Please note that sc-542 is going to be discontinued at the end of this year. We recommend you try one of our suggested replacement mouse monoclonal antibodies against ERalpha. Please contact our Technical Service Department and we can send you a free sample to test of the best antibody for your research needs. Please call us at (800) 457-3801 option 2, email scbt@scbt.com, or connect with us on live chat.
Répondue par: Technical Support
Date de publication: 2016-10-05
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Rated 5 de 5 de par Work in IHCWorked well in Immunohistochemistry with a dillution of 1/500
Date de publication: 2022-07-07
Rated 5 de 5 de par well in WBwo use it for years,and test it in some cell and tissues.for this result,wo test it in human cell
Date de publication: 2016-12-05
Rated 4 de 5 de par goodworks well in frozen section of mammary gland, fixation with 4% PFA 2h
Date de publication: 2016-11-30
Rated 3 de 5 de par not very good in ChIPit did not work well in ChIP using mouse thymocyte cells
Date de publication: 2016-11-29
Rated 5 de 5 de par Great for immunohistochemistryWorked very well in immunohistochemistry (1:100) on murine mammary gland tissue (paraffin embedded with antigen retrieval)
Date de publication: 2016-11-22
Rated 5 de 5 de par Antibody was used on mouse pancreas for immunoblotAntibody was used on mouse pancreas for immunoblot at 1:200 giving excellent result.
Date de publication: 2016-02-02
Rated 5 de 5 de par The antibody effect is very clearThe antibody effect is very clear, the background is low, with special perfect!
Date de publication: 2015-10-14
Rated 5 de 5 de par Able to publish using the antibody for WBAble to publish using the antibody for WB and IHC in mouse sample.
Date de publication: 2015-09-16
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Anticorps ERα (MC-20) est évalué 4.8 de 5 de 20.
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