ER81 Double Nickase Plasmid (h): sc-401594-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ER81 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ER81 Double Nickase Plasmid (h) and ER81 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ETV1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ER81 Double Nickase Plasmid (h) | sc-401594-NIC | 20 µg | $410.00 | |||
ER81 Double Nickase Plasmid (h2) | sc-401594-NIC-2 | 20 µg | $410.00 |
ETV1 encodes the ETS family transcription factor ER81, a sequence-specific DNA-binding protein that coordinates gene expression programs controlling cell fate decisions, proliferation, and differentiation. ER81 integrates signals from MAPK/ERK and receptor tyrosine kinase pathways to modulate transcriptional outputs involved in neuronal development and tissue patterning. Dysregulated ETV1 activity has been linked to oncogenic transcriptional networks, including gene fusions and aberrant enhancer-driven expression in multiple tumor contexts, making it a widely used model for studying lineage programs and signaling-dependent transcription. In human cells, ER81 target regulation provides a tractable readout for investigating ETS-mediated promoter/enhancer selection, cofactor recruitment, and chromatin-dependent transcriptional control.
ER81 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ETV1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ETV1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ETV1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ETV1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.