Date published: 2026-4-28

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ε Tubulin Antibody (5F3B7): sc-517236

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Datasheets
  • ε Tubulin Antibody (5F3B7) is a mouse monoclonal IgG1 ε Tubulin antibody provided at 100 µg/ml
  • raised against a recombinant protein corresponding to amino acids 314-472 of ε Tubulin of human origin
  • recommended for detection of ε Tubulin of human origin by WB, IP, IF, IHC(P), FCM and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ε Tubulin Antibody (5F3B7). This work is in progress.
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    ε Tubulin Antibody (5F3B7) is a mouse monoclonal IgG1 antibody that detects the epsilon Tubulin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-epsilon Tubulin antibody (5F3B7) is available as the non-conjugated format. Epsilon Tubulin is a crucial component of the microtubule cytoskeleton, which plays a vital role in maintaining cell shape, enabling intracellular transport, and facilitating cell division. Epsilon Tubulin exists as one of five distinct forms of tubulin, alongside alpha, beta, gamma, and delta tubulin, and specifically localizes to the pericentriolar material of the centrosome. This localization proves essential for proper organization and function of microtubules during cell division, particularly in mitotic spindle formation. Epsilon Tubulin′s unique cell-cycle-specific pattern of localization allows association with one centrosome in a newly duplicated pair and later with both, highlighting importance in accurate chromosome segregation. Anti-epsilon Tubulin antibody (5F3B7) effectively detects this protein across various applications, making epsilon Tubulin antibody (5F3B7) an invaluable tool for researchers studying cytoskeleton dynamics and implications in cellular processes and diseases.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    ε Tubulin Antibody (5F3B7) References:

    1. Presence of the betaII isotype of tubulin in the nuclei of cultured mesangial cells from rat kidney.  |  Walss, C., et al. 1999. Cell Motil Cytoskeleton. 42: 274-84. PMID: 10223634
    2. Identification of betaIII- and betaIV-tubulin isotypes in cold-adapted microtubules from Atlantic cod (Gadus morhua): antibody mapping and cDNA sequencing.  |  Modig, C., et al. 1999. Cell Motil Cytoskeleton. 42: 315-30. PMID: 10223637
    3. Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function.  |  Chang, P. and Stearns, T. 2000. Nat Cell Biol. 2: 30-5. PMID: 10620804
    4. Tubulin immunoreactive neuronal intranuclear inclusions in the human brain.  |  Woulfe, J. and Munoz, D. 2000. Neuropathol Appl Neurobiol. 26: 161-71. PMID: 10840279
    5. Long-lost relatives reappear: identification of new members of the tubulin superfamily.  |  Dutcher, SK. 2003. Curr Opin Microbiol. 6: 634-40. PMID: 14662361
    6. Alpha-, beta-, and gamma-tubulins: sequence comparisons and structural constraints.  |  Burns, RG. 1991. Cell Motil Cytoskeleton. 20: 181-9. PMID: 1773446
    7. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.  |  Zheng, Y., et al. 1991. Cell. 65: 817-23. PMID: 1904010
    8. Delta and epsilon tubulin in mammalian development.  |  Stathatos, GG., et al. 2021. Trends Cell Biol. 31: 774-787. PMID: 33867233
    9. Epsilon tubulin is an essential determinant of microtubule-based structures in male germ cells.  |  Stathatos, GG., et al. 2024. EMBO Rep. 25: 2722-2742. PMID: 38773322
    10. Invited review: the role of nucleotide triphosphate in actin and tubulin assembly and function.  |  Weisenberg, RC. 1981. Cell Motil. 1: 485-97. PMID: 6756643
    11. Multiple forms of tubulin: different gene products and covalent modifications.  |  Ludueña, RF. 1998. Int Rev Cytol. 178: 207-75. PMID: 9348671
    12. Expression of amino- and carboxyl-terminal gamma- and alpha-tubulin mutants in cultured epithelial cells.  |  Leask, A. and Stearns, T. 1998. J Biol Chem. 273: 2661-8. PMID: 9446570

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    ε Tubulin Antibody (5F3B7)

    sc-517236
    100 µg/ml
    $322.00

    What is the recommended storage condition for ε Tubulin (5F3B7): sc-517236?

    Asked by: Germaine
    Thank you for your question. We recommend storing the antibody at 4°C.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 5 out of 5 by from Good for Western BlotAntibody detects human recombinant ε Tubulin and ε Tubulin transfected lysate by Western blot. -SCBT QC
    Date published: 2023-09-14
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    ε Tubulin Antibody (5F3B7) is rated 5.0 out of 5 by 1.
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