Eps8L1 Double Nickase Plasmid (h): sc-406032-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Eps8L1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Eps8L1 Double Nickase Plasmid (h) and Eps8L1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPS8L1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eps8L1 Double Nickase Plasmid (h) | sc-406032-NIC | 20 µg | $410.00 | |||
Eps8L1 Double Nickase Plasmid (h2) | sc-406032-NIC-2 | 20 µg | $410.00 |
EPS8L1 encodes Eps8L1, a member of the EPS8 family of actin-regulatory proteins that couple receptor-mediated signaling to cytoskeletal remodeling. Eps8L1 is implicated in pathways governing actin filament dynamics, membrane protrusion formation, and cell shape changes that support adhesion and motility. Through interactions with signaling and cytoskeletal partners, EPS8L1 can influence processes such as endocytosis and migration-associated remodeling of the cortical actin network. Dysregulated actin control and motility programs are frequently associated with invasive phenotypes and aberrant tissue organization, making EPS8L1 a relevant target for mechanistic studies of cell movement and cytoskeletal signaling.
Eps8L1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPS8L1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPS8L1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPS8L1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPS8L1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.