Eps15 Antibody (G-3): sc-374578
- Eps15 Antibody (G-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-896 of Eps15 of human origin
- recommended for detection of Eps15 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Eps15 Antibody (G-3) for WB applications. These reagents are now offered in bundles with Eps15 Antibody (G-3) (see ordering information below).
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Eps15 Antibody (G-3) is a mouse monoclonal IgG1 antibody that detects Eps15 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Eps15 plays a crucial role in regulating cellular processes, particularly in receptor tyrosine kinase (RTK) signaling pathways, which are essential for normal cell growth and differentiation. Misregulation of these pathways can lead to cellular transformation and cancer, highlighting the importance of understanding Eps15′s function. Eps15 is primarily located in the cytoplasm and is involved in endocytosis, participating in the internalization of activated receptors and modulating signal transduction. Eps15′s interaction with other proteins, such as Crk, through a conserved proline-rich domain, further underscores Eps15′s significance in cellular signaling networks. Additionally, Eps15 undergoes tyrosine phosphorylation upon EGF stimulation, a modification critical for promoting cell proliferation and transformation. Anti-Eps15 antibody (G-3) serves as an invaluable tool for researchers exploring RTK signaling mechanisms and their implications in cancer biology.
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Eps15 Antibody (G-3) References:
- Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1. | Chi, S., et al. 2008. Mol Biol Cell. 19: 3564-75. PMID: 18524853
- Distinct recruitment of Eps15 via Its coiled-coil domain is required for efficient down-regulation of the met receptor tyrosine kinase. | Parachoniak, CA. and Park, M. 2009. J Biol Chem. 284: 8382-94. PMID: 19109251
- p38-Mediated phosphorylation of Eps15 endocytic adaptor protein. | Zhou, Y., et al. 2014. FEBS Lett. 588: 131-7. PMID: 24269888
- UIM domain-dependent recruitment of the endocytic adaptor protein Eps15 to ubiquitin-enriched endosomes. | Gucwa, AL. and Brown, DA. 2014. BMC Cell Biol. 15: 34. PMID: 25260758
- Over-expression of EPS15 is a favorable prognostic factor in breast cancer. | Dai, X., et al. 2015. Mol Biosyst. 11: 2978-85. PMID: 26289382
- Increased Eps15 homology domain 1 and RAB11FIP3 expression regulate breast cancer progression via promoting epithelial growth factor receptor recycling. | Tong, D., et al. 2017. Tumour Biol. 39: 1010428317691010. PMID: 28215104
- Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition. | Lapierre, LA., et al. 2017. Mol Biol Cell. 28: 1088-1100. PMID: 28228550
- Cellular functions and intrinsic attributes of the ATP-binding Eps15 homology domain-containing proteins. | Bhattacharyya, S. and Pucadyil, TJ. 2020. Protein Sci. 29: 1321-1330. PMID: 32223019
- Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. | Gu, L., et al. 2023. Pharmacol Res. 192: 106789. PMID: 37149115
- AP-2/Eps15 interaction is required for receptor-mediated endocytosis. | Benmerah, A., et al. 1998. J Cell Biol. 140: 1055-62. PMID: 9490719
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eps15 Antibody (G-3) | sc-374578 | 200 µg/ml | $322.00 | |||
Eps15 Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-540479 | 200 µg Ab; 10 µg BP | $361.00 | |||
Eps15 Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-542150 | 200 µg Ab; 20 µg BP | $361.00 |