Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EpoR Double Nickase Plasmid (h): sc-400442-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EpoR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EpoR Double Nickase Plasmid (h) and EpoR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPOR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EpoR Antibody (D-5): sc-365662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EpoR Double Nickase Plasmid (h) | sc-400442-NIC | 20 µg | $410.00 | |||
EpoR Double Nickase Plasmid (h2) | sc-400442-NIC-2 | 20 µg | $410.00 |
EPOR encodes the erythropoietin receptor (EpoR), a type I cytokine receptor that governs erythroid progenitor survival, proliferation, and differentiation in response to erythropoietin. Ligand-induced receptor dimerization activates JAK2 and downstream STAT5, PI3K–AKT, and RAS–MAPK signaling, coordinating transcriptional programs that support erythropoiesis and cellular stress responses. Dysregulated EPOR signaling has been linked to altered red blood cell production and aberrant growth signaling in hematologic and other contexts, making it a useful node for studying cytokine receptor biology. EPOR is also used as a model receptor to dissect receptor trafficking, signal attenuation, and cross-talk with hypoxia and iron homeostasis pathways.
EpoR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPOR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPOR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPOR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPOR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.