EpoR Antibody (D-5): sc-365662

EpoR Antibody (D-5) is a mouse monoclonal IgG3 kappa light chain antibody that detects EpoR of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EpoR (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The erythropoietin receptor (EpoR) plays a crucial role in erythropoiesis, as EpoR mediates the effects of erythropoietin (Epo), a key hormone in red blood cell production. EpoR is a 507 amino acid transmembrane protein that undergoes homodimerization upon Epo binding, which is essential for initiating downstream signaling pathways that lead to cell proliferation and survival. Notably, EpoR shares significant structural similarities with the interleukin 2 receptor beta-chain subunit, particularly in the conserved box 1 and box 2 domains of their cytoplasmic regions, which are critical for receptor function. These conserved domains facilitate the coupling of ligand binding to the induction of tyrosine phosphorylation, a key step in signal transduction. Additionally, the membrane proximal region of EpoR is vital for the recruitment of Janus kinase 2 (JAK2), further underscoring the importance of EpoR′s structure in functional role. The presence of various cross-linked complexes and differing ligand affinities indicates that EpoR may operate as part of a multireceptor complex, highlighting EpoR′s intricate involvement in cellular signaling and the regulation of erythropoiesis.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EpoR Antibody (D-5) References:

1. Anti-Epo receptor antibodies do not predict Epo receptor expression.  |  Elliott, S., et al. 2006. Blood. 107: 1892-5. PMID: 16249375
2. Critical cytoplasmic region of the interleukin 6 signal transducer gp130 is conserved in the cytokine receptor family.  |  Murakami, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 11349-53. PMID: 1662392
3. The cytoplasmic region of the erythropoietin receptor contains nonoverlapping positive and negative growth-regulatory domains.  |  D'Andrea, AD., et al. 1991. Mol Cell Biol. 11: 1980-7. PMID: 1848667
4. Structural design and molecular evolution of a cytokine receptor superfamily.  |  Bazan, JF. 1990. Proc Natl Acad Sci U S A. 87: 6934-8. PMID: 2169613
5. EpoR-tdTomato-Cre mice enable identification of EpoR expression in subsets of tissue macrophages and hematopoietic cells.  |  Zhang, H., et al. 2021. Blood. 138: 1986-1997. PMID: 34098576
6. EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis.  |  Hidalgo, D., et al. 2021. Nat Commun. 12: 7334. PMID: 34921133
7. Identification of Two Novel EPOR Gene Variants in Primary Familial Polycythemia: Case Report and Literature Review.  |  Lo Riso, L., et al. 2022. Genes (Basel). 13: PMID: 36292571
8. Erythropoietin regulates energy metabolism through EPO-EpoR-RUNX1 axis.  |  Yin, W., et al. 2024. Nat Commun. 15: 8114. PMID: 39284834
9. Characterization of three erythropoietin (Epo)-binding proteins in various human Epo-responsive cell lines and in cells transfected with human Epo-receptor cDNA.  |  Takahashi, T., et al. 1995. Blood. 85: 106-14. PMID: 7803787
10. Activation and inhibition of erythropoietin receptor function: role of receptor dimerization.  |  Watowich, SS., et al. 1994. Mol Cell Biol. 14: 3535-49. PMID: 8196600
11. Inactivation of erythropoietin receptor function by point mutations in a region having homology with other cytokine receptors.  |  Miura, O., et al. 1993. Mol Cell Biol. 13: 1788-95. PMID: 8382775
12. Structure, function, and activation of the erythropoietin receptor.  |  Youssoufian, H., et al. 1993. Blood. 81: 2223-36. PMID: 8481505

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EpoR Antibody (D-5) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-EpoR Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EpoR Antibody (D-5) Specifications

Alternate Product Name: EpoR

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 491-513

Epitope Target: EpoR

Epitope Species: human

Product Reactivity/Detection: EpoR

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for EpoR Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin