Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EPHX2 Double Nickase Plasmid (h): sc-402095-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- sEH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- sEH Double Nickase Plasmid (h) and sEH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPHX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: sEH Antibody (A-5): sc-166961
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EPHX2 Double Nickase Plasmid (h) | sc-402095-NIC | 20 µg | $410.00 | |||
sEH Double Nickase Plasmid (h2) | sc-402095-NIC-2 | 20 µg | $410.00 |
EPHX2 encodes soluble epoxide hydrolase (sEH), a bifunctional enzyme that converts lipid epoxides derived from polyunsaturated fatty acids into corresponding diols, thereby modulating the balance of epoxyeicosatrienoic acids and related epoxy-fatty acids. Through this epoxide hydrolysis step, EPHX2 influences eicosanoid and oxylipin signaling pathways that regulate vascular tone, inflammatory signaling, oxidative stress responses, and cellular metabolism. Altered EPHX2 activity and oxylipin profiles have been associated with cardiometabolic phenotypes, including hypertension, insulin resistance, and atherosclerosis-related processes, as well as neuroinflammatory and pain-related signaling. In biomedical research, EPHX2 is commonly studied in the context of lipid mediator biology, endothelial and immune cell function, and stress-response pathways that couple metabolic inputs to inflammatory outputs.
sEH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPHX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPHX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPHX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPHX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.