ephrin-A5 Antibody (RR-7) recommended for detection of ephrin-A5 of mouse, rat and human origin by WB, IP and ELISA

ephrin-A5 Antibody (RR-7): sc-81945

ephrin-A5 Antibody (RR-7) is rated 3.3 out of 5 by 3.
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Datasheets

    • Anti-ephrin-A5 Antibody (RR-7) is a mouse monoclonal IgG2a (kappa light chain) ephrin-A5 antibody provided at 100 µg/ml
    • raised against a partial recombinant protein mapping within amino acid 114-203 of ephrin-A5 of human origin
    • recommended for detection of ephrin-A5 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ephrin-A5 (RR-7): sc-81945.

Do you the epitope against which this Mab is directed?

Asked by: Patricia Maness
Thank you for your question. This antibody was made against a recombinant protein mapping within amino acids 114-203 of human ephrin-A5. The exact epitope has not been mapped.
Answered by: Technical Support
Date published: 2019-01-26

For Western Blot, is it recommended to use denatured or non-denatured conditions with ephrin-A5 (RR-7): sc-81945 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from not specific for Ephrin-A5 This antibody gave a strong band around 30 Kd. However, it is not specific for Ephrin-A5 as EphrinA5 null mouse tissue (in which a big portion of Ephrin-A5 protein has bee deleted) showed the same band.
Date published: 2018-07-24
Rated 5 out of 5 by from Good antibody IF gives specific staining. -- tested on cryosections from 4% PFA fixed mouse embryonic brain. Use 1:200 with 5 min antigen retrieval (citric buffer, PH 6.0).
Date published: 2017-10-11
Rated 4 out of 5 by from Band at expected molecular weight in western Band at expected molecular weight in western blot analysis of ephrin-A5 expression in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2013-10-09
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