Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EP4 Double Nickase Plasmid (h): sc-401146-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EP4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EP4 Double Nickase Plasmid (h) and EP4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTGER4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EP4 Antibody (C-4): sc-55596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EP4 Double Nickase Plasmid (h) | sc-401146-NIC | 20 µg | $410.00 | |||
EP4 Double Nickase Plasmid (h2) | sc-401146-NIC-2 | 20 µg | $410.00 |
PTGER4 encodes the human prostaglandin E2 receptor EP4, a G protein–coupled receptor that primarily signals through Gs to elevate cAMP and activate PKA/CREB-dependent transcriptional programs. EP4 also engages β-arrestin– and PI3K/AKT- and ERK/MAPK-linked signaling, coordinating regulation of inflammatory responses, vascular tone, epithelial barrier function, and cell migration. In immune and stromal compartments, EP4 modulates cytokine production and leukocyte trafficking downstream of COX-derived PGE2, integrating prostanoid signaling with broader immunoregulatory networks. Dysregulated PTGER4/EP4 activity has been associated with chronic inflammatory phenotypes and altered tissue homeostasis, supporting its use as a mechanistic node in studies of inflammation-linked disease biology.
EP4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTGER4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTGER4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTGER4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTGER4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.