Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ENT1 Double Nickase Plasmid (h): sc-401603-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ENT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ENT1 Double Nickase Plasmid (h) and ENT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC29A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ENT1 Antibody (F-12): sc-377283
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENT1 Double Nickase Plasmid (h) | sc-401603-NIC | 20 µg | $410.00 | |||
ENT1 Double Nickase Plasmid (h2) | sc-401603-NIC-2 | 20 µg | $410.00 |
Human SLC29A1 encodes equilibrative nucleoside transporter 1 (ENT1), a bidirectional plasma membrane transporter that mediates Na⁺-independent uptake and efflux of purine and pyrimidine nucleosides. ENT1 helps regulate intracellular nucleoside pools that support the purine/pyrimidine salvage pathways, DNA/RNA synthesis, and cellular energy homeostasis through adenosine transport. By shaping extracellular adenosine availability, ENT1 influences purinergic signaling and downstream responses such as inflammatory modulation and vascular tone. Altered SLC29A1 activity has been linked in the literature to neurobehavioral phenotypes and tissue-specific dysregulation of adenosine signaling, motivating mechanistic studies in neuronal, immune, and barrier cell models.
ENT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC29A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC29A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC29A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC29A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.