Date published: 2026-4-5

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Endoproteinase Lys-C (CAS 72561-05-8)

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Alternate Names:
Achromopeptidase
Application:
Endoproteinase Lys-C is a highly specific endopeptidase that hydrolyzes lysyl bonds
CAS Number:
72561-05-8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Endoproteinase Lys-C is a highly specific enzyme found in the bacterium Lysobacter enzymogenes, known for its precision in cleaving peptide bonds at the carboxyl side of lysine residues. This attribute makes it an invaluable tool in proteomics and protein sequencing research, facilitating the detailed analysis of protein structures and functions. By breaking down proteins into smaller, manageable peptides without affecting other amino acid sequences, Endoproteinase Lys-C enables researchers to decipher the complex puzzle of protein interactions and post-translational modifications. Its ability to function in a variety of conditions, including different pH levels and temperatures, further enhances its utility in experimental setups. Through its targeted action, this enzyme supports advancements in understanding the molecular underpinnings of various biological processes and diseases, contributing to the development of novel research methodologies and the exploration of protein dynamics.


Endoproteinase Lys-C (CAS 72561-05-8) References

  1. An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization.  |  Sekar, R., et al. 2003. Appl Environ Microbiol. 69: 2928-35. PMID: 12732568
  2. Lytic enzyme, labiase for a broad range of Gram-positive bacteria and its application to analyze functional DNA/RNA.  |  Niwa, T., et al. 2005. J Microbiol Methods. 61: 251-60. PMID: 15722152
  3. Evaluation of enzymatic cell treatments for application of CARD-FISH to methanogens.  |  Kubota, K., et al. 2008. J Microbiol Methods. 72: 54-9. PMID: 18022717
  4. Comparison of point-of-care-compatible lysis methods for bacteria and viruses.  |  Heiniger, EK., et al. 2016. J Microbiol Methods. 128: 80-87. PMID: 27424294
  5. A comparison of different pre-lysis methods and extraction kits for recovery of Streptococcus agalacticae (Lancefield group B Streptococcus) DNA from whole blood.  |  Burke, RM., et al. 2016. J Microbiol Methods. 129: 103-108. PMID: 27546716
  6. Complete genome sequence and expression profile of the commercial lytic enzyme producer Lysobacter enzymogenes M497-1.  |  Takami, H., et al. 2017. DNA Res. 24: 169-177. PMID: 28065880
  7. Rapid isolation of DNA from Actinomyces.  |  Barsotti, O., et al. 1987. Ann Inst Pasteur Microbiol. 138: 529-36. PMID: 3125848
  8. Achromopeptidase for rapid lysis of oral anaerobic gram-positive rods.  |  Barsotti, O., et al. 1988. Oral Microbiol Immunol. 3: 86-8. PMID: 3268757
  9. Serogrouping single colonies of beta-hemolytic streptococci with achromopeptidase extraction.  |  Slifkin, M. and Cumbie, R. 1987. J Clin Microbiol. 25: 1555-6. PMID: 3305569
  10. Detection of an alkene monooxygenase in vinyl chloride-oxidizing bacteria with GeneFISH.  |  Richards, PM. and Mattes, TE. 2021. J Microbiol Methods. 181: 106147. PMID: 33493490
  11. Nitrocellulose-bound achromopeptidase for point-of-care nucleic acid tests.  |  Chondrogiannis, G., et al. 2021. Sci Rep. 11: 6140. PMID: 33731748
  12. Achromopeptidase for lysis of anaerobic gram-positive cocci.  |  Ezaki, T. and Suzuki, S. 1982. J Clin Microbiol. 16: 844-6. PMID: 6759529
  13. Lysis of Streptococcus mutans with achromopeptidase and release of osmotically fragile bodies.  |  Ota, F., et al. 1982. Microbiol Immunol. 26: 957-63. PMID: 6761559
  14. Purification, staphylolytic activity, and cleavage sites of alpha-lytic protease from Achromobacter lyticus.  |  Li, S., et al. 1997. J Biochem. 122: 772-8. PMID: 9399581
  15. Rapid lysis of gram-positive cocci for pulsed-field gel electrophoresis using achromopeptidase.  |  Leonard, RB. and Carroll, KC. 1997. Diagn Mol Pathol. 6: 288-91. PMID: 9458388

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Endoproteinase Lys-C, 5 µg

sc-360250
5 µg
$87.00

Endoproteinase Lys-C, 20 µg

sc-360250C
20 µg
$235.00

Endoproteinase Lys-C, 100 µg

sc-360250A
100 µg
$449.00

Endoproteinase Lys-C, 660 µg

sc-360250B
660 µg
$714.00

Endoproteinase Lys-C, 3 mg

sc-360250D
3 mg
$1125.00

What is the source of this LysC? Lysobacter or achromobacter? or other? and it is native or recombinant?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-28

How long can lys-c (sc-360250B) store at -20 after reconstitution?

Asked by: xy123
Thank you for your question. We have a one year warranty on all of our chemicals, we do not have the exact stability once introduced to a solution, we recommend immediate use rather than long term storage after reconstitution. If you have any further questions, please contact technical support.
Answered by: Technical Service 4
Date published: 2020-11-13

Is the Endoproteinase Lys-C, 660 µg (sc-360250B) aliquoted in 20ug bottle or just in one big bottle?

Asked by: Junephone
Thank you for your question. It is provided as lyophilized in 1 bottle.
Answered by: Tech Service
Date published: 2020-03-16

What is the recommended reconstitution solution?

Asked by: Analyst
Thank you for your question. The recommended solvent for creating a working solution of Endoproteinase Lys-C is 25 mM Tris/HCl, 300 mM NaCl, 1mM EDTA pH 8.0.
Answered by: Tech Support Europe
Date published: 2019-06-28

What are the storage conditions after reconstitution?

Asked by: chemsd02
Thank you for your question. sc-360250 (Lysyl endopeptidase) should be aliquotted and stored at -20ºC. It can be stored at 4ºC for a few days if it's being used frequently.
Answered by: Chemical Support 10
Date published: 2018-04-05

Can you please confirm the grade of sc-360250? We would need the sequencing grade.

Asked by: two2igm05
Thank you for your question. Lysyl endopeptidase, sc-360250, is sequencing grade.
Answered by: TechService7
Date published: 2018-05-31

Wie schnell ist Katalognr. sc-360250D verfügbar?

Asked by: Silke
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Rated 5 out of 5 by from Various publications cite the use of LysylVarious publications cite the use of Lysyl endopeptidase, an enzyme that hydrolyzes lysyl peptide bonds of non-terminal amino acids, in research as a standard laboratory reagent for enzyme digestion. This is useful to study the primary structure of proteins. -SCBT Publication Review
Date published: 2015-04-23
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Endoproteinase Lys-C is rated 5.0 out of 5 by 1.
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