Endomucin Antibody (V.7C7): sc-65495

Endomucin Antibody (V.7C7) is a rat monoclonal IgG2a antibody that detects Endomucin protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). Anti-Endomucin antibody (V.7C7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Endomucin, also referred to as EMCN or Mucin-14, is a 261 amino acid protein characterized by a transmembrane sequence and numerous glycosylation sites, which are crucial for function. The presence of O-linked glycans on serine and threonine residues forces Endomucin into an extended conformation, enhancing interactions with other molecules. Endomucin is predominantly expressed in vascular tissues such as heart, kidney, and lung, and exists in two forms: an unprocessed precursor peptide and a processed variant known as Endomucin 2, which consists of 241 amino acids. In mice, Endomucin serves as an endothelial antigen found in venous endothelium and capillaries, but is absent in arterial endothelium. Notably, Endomucin expression is upregulated during endothelial cell proliferation and in response to tumor-conditioned media or angiogenic factors like basic fibroblast growth factor and tumor necrosis factor-alpha. Furthermore, Endomucin overexpression has been shown to inhibit adhesion and aggregation of hematopoietic cells, indicating a potential role in facilitating detachment of these cells from endothelium during early stages of hematopoiesis.

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Endomucin Antibody (V.7C7) References:

1. Identification of human endomucin-1 and -2 as membrane-bound O-sialoglycoproteins with anti-adhesive activity.  |  Kinoshita, M., et al. 2001. FEBS Lett. 499: 121-6. PMID: 11418125
2. Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion.  |  Ueno, M., et al. 2001. Biochem Biophys Res Commun. 287: 501-6. PMID: 11554756
3. Human endomucin is an endothelial marker.  |  Liu, C., et al. 2001. Biochem Biophys Res Commun. 288: 129-36. PMID: 11594763
4. The dimensions of the T lymphocyte glycoprotein leukosialin and identification of linear protein epitopes that can be modified by glycosylation.  |  Cyster, JG., et al. 1991. EMBO J. 10: 893-902. PMID: 1706994
5. Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression.  |  Kanki, Y., et al. 2011. EMBO J. 30: 2582-95. PMID: 21666600
6. The α1,3-fucosyltransferase FUT7 regulates IL-1β-induced monocyte-endothelial adhesion via fucosylation of endomucin.  |  Zhang, J., et al. 2018. Life Sci. 192: 231-237. PMID: 29138114
7. Endomucin inhibits VEGF-induced endothelial cell migration, growth, and morphogenesis by modulating VEGFR2 signaling.  |  Park-Windhol, C., et al. 2017. Sci Rep. 7: 17138. PMID: 29215001
8. Human adult HSCs can be discriminated from lineage-committed HPCs by the expression of endomucin.  |  Reckzeh, K., et al. 2018. Blood Adv. 2: 1628-1632. PMID: 29986855
9. Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.  |  Hu, Z., et al. 2020. Cells. 9: PMID: 32517158
10. Biochemical characterization and molecular cloning of a novel endothelial-specific sialomucin.  |  Morgan, SM., et al. 1999. Blood. 93: 165-75. PMID: 9864158

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Endomucin Antibody (V.7C7) has 426 citations in a variety of scientific publications.

Primary antibodies like Anti-Endomucin Antibody (V.7C7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Endomucin Antibody (V.7C7) Specifications

Alternate Product Name: Endomucin

Product Clone Name: V.7C7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: Endomucin

Epitope Species: mouse

Product Reactivity/Detection: Endomucin

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 426 citations in various scientific publications

Available Conjugates/Forms for Endomucin Antibody (V.7C7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin