EN2/Engrailed 2 Antibody (1E1): sc-293311

EN-2 Antibody (1E1) is a mouse monoclonal IgG2a kappa light chain antibody that detects EN-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-EN-2 antibody (1E1) is available as the non-conjugated monoclonal isotype antibody. EN-2 protein, encoded by the engrailed-2 gene (EN2), plays a crucial role in the development of the midbrain and cerebellum, as well as in the dorsal/ventral patterning of limbs and the formation of the apical ectodermal ridge. EN-2 protein is essential for proper segmentation during development, as evidenced by murine homolog′s involvement in the formation of posterior compartments in Drosophila. In humans, EN-1 and EN-2 are homeodomain-containing proteins that are vital for the regulation of pattern formation in the central nervous system. Notably, mutations in mouse homologs EN-1 and EN-2 can lead to various developmental defects, often resulting in lethality. EN-1 is predominantly expressed in dopaminergic neurons within the substantia nigra and ventral tegmentum, and EN-1 and EN-2 regulate the expression of alpha-synuclein, a gene associated with Parkinson′s disease. During early brain development, mouse EN-2 is expressed broadly across the mid-hindbrain region, and EN-2 expression in mouse myoblasts has been linked to cerebellar hypoplasia, highlighting EN-2 protein′s significance in both neural and muscular development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EN2/Engrailed 2 Antibody (1E1) References:

1. Fibroblast growth factor-8 expression is regulated by intronic engrailed and Pbx1-binding sites.  |  Gemel, J., et al. 1999. J Biol Chem. 274: 6020-6. PMID: 10026229
2. Two Pax2/5/8-binding sites in Engrailed2 are required for proper initiation of endogenous mid-hindbrain expression.  |  Li Song, D. and Joyner, AL. 2000. Mech Dev. 90: 155-65. PMID: 10640701
3. FGF10 can induce Fgf8 expression concomitantly with En1 and R-fng expression in chick limb ectoderm, independent of its dorsoventral specification.  |  Ohuchi, H., et al. 1999. Dev Growth Differ. 41: 665-73. PMID: 10646796
4. A role for Engrailed-2 in determination of skeletal muscle physiologic properties.  |  Degenhardt, K. and Sassoon, DA. 2001. Dev Biol. 231: 175-89. PMID: 11180961
5. Fate of midbrain dopaminergic neurons controlled by the engrailed genes.  |  Simon, HH., et al. 2001. J Neurosci. 21: 3126-34. PMID: 11312297
6. Agenesis of the mesencephalon and metencephalon with cerebellar hypoplasia: putative mutation in the EN2 gene--report of 2 cases in early infancy.  |  Sarnat, HB., et al. 2002. Pediatr Dev Pathol. 5: 54-68. PMID: 11815869
7. T-cell acute lymphoblastic leukemia--the associated gene SCL/tal codes for a 42-Kd nuclear phosphoprotein.  |  Goldfarb, AN., et al. 1992. Blood. 80: 2858-66. PMID: 1450410
8. Drosophila engrailed can substitute for mouse Engrailed1 function in mid-hindbrain, but not limb development.  |  Hanks, MC., et al. 1998. Development. 125: 4521-30. PMID: 9778510