EN1/Engrailed 1 Antibody (3-RY3): sc-134328
- EN1/Engrailed 1 Antibody (3-RY3) is a mouse monoclonal mouse IgG2a κ κ provided at 100 µg/ml
- raised against recombinant EN-1 protein of human origin
- recommended for detection of EN-1 of mouse, rat and human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for EN1/Engrailed 1 Antibody (3-RY3). This work is in progress.
EN-1 Antibody (3-RY3) is a mouse monoclonal IgG2a antibody that detects engrailed-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Engrailed-1 (EN-1) is a crucial transcription factor involved in the development of the midbrain and cerebellum, as well as in the dorsal/ventral patterning of limbs and the formation of the apical ectodermal ridge. EN-1 (3-RY3) antibody enables research into segmentation processes during embryonic development, helping establish the anterior-posterior axis in developing tissues. In humans, EN-1, along with EN-2 homolog, plays a significant role in the patterning of the central nervous system, and mutations in these genes can lead to severe developmental defects, often resulting in lethality. EN-1 is predominantly expressed in dopaminergic neurons located in the substantia nigra and ventral tegmentum, areas critically involved in motor control and implicated in neurodegenerative diseases such as Parkinson′s disease. The regulation of alpha-synuclein expression by EN-1 and EN-2 further highlights the importance of studying these proteins in understanding molecular mechanisms underlying such disorders. Anti-EN-1 antibody (3-RY3) serves as an invaluable tool for researchers investigating developmental biology and neurodegeneration.
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EN1/Engrailed 1 Antibody (3-RY3) References:
- The Engrailed-1 Gene Stimulates Brown Adipogenesis. | Zhang, C., et al. 2016. Stem Cells Int. 2016: 7369491. PMID: 27148369
- Engrailed 1 overexpression as a potential prognostic marker in quintuple-negative breast cancer. | Kim, YJ., et al. 2018. Cancer Biol Ther. 19: 335-345. PMID: 29333926
- Engrailed-1 induces long-lasting behavior benefit in an experimental Parkinson primate model. | Thomasson, N., et al. 2019. Mov Disord. 34: 1082-1084. PMID: 31077447
- Preventing Engrailed-1 activation in fibroblasts yields wound regeneration without scarring. | Mascharak, S., et al. 2021. Science. 372: PMID: 33888614
- Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation. | Györfi, AH., et al. 2021. J Exp Med. 218: PMID: 34259830
- ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneurons. | Lebœuf, M., et al. 2023. EMBO Rep. 24: e56525. PMID: 37534581
- Engrailed-1 Promotes Pancreatic Cancer Metastasis. | Xu, J., et al. 2024. Adv Sci (Weinh). 11: e2308537. PMID: 38110836
- Engrailed 1 deficiency induces changes in ciliogenesis during human neuronal differentiation. | Hembach, S., et al. 2024. Neurobiol Dis. 194: 106474. PMID: 38518837
- Cannabinoid type-1 receptor signaling in dopaminergic Engrailed-1 expressing neurons modulates motivation and depressive-like behavior. | Baddenhausen, S., et al. 2024. Front Mol Neurosci. 17: 1379889. PMID: 38660383
- Engrailed-1 as a target of the Wnt-1 signalling pathway in vertebrate midbrain development. | Danielian, PS. and McMahon, AP. 1996. Nature. 383: 332-4. PMID: 8848044
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EN1/Engrailed 1 Antibody (3-RY3) | sc-134328 | 100 µg/ml | $339.00 |