EMP-2 Double Nickase Plasmid (m): sc-420166-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMP-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EMP-2 Double Nickase Plasmid (m) and EMP-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Emp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMP-2 Double Nickase Plasmid (m) | sc-420166-NIC | 20 µg | $410.00 | |||
EMP-2 Double Nickase Plasmid (m2) | sc-420166-NIC-2 | 20 µg | $410.00 |
Emp2 encodes epithelial membrane protein 2 (EMP-2), a tetraspan membrane-associated protein enriched in epithelial tissues that helps organize membrane microdomains and modulate cell–cell and cell–matrix interactions. In mouse cells, EMP-2 has been linked to regulation of integrin trafficking and signaling, influencing focal adhesion dynamics, cytoskeletal remodeling, and downstream pathways that shape adhesion, migration, and tissue architecture. Altered EMP-2 expression has been associated with changes in epithelial differentiation and barrier properties, making it relevant for studying mechanisms that underlie abnormal tissue remodeling. Emp2 is therefore a useful target in models interrogating membrane organization, adhesion-dependent signaling, and epithelial stress responses.
EMP-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Emp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Emp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Emp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Emp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.