EMMPRIN/CD147 Double Nickase Plasmid (h): sc-400589-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EMMPRIN/CD147 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EMMPRIN/CD147 Double Nickase Plasmid (h) and EMMPRIN/CD147 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BSG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EMMPRIN/CD147 Antibody (8D6): sc-21746
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMMPRIN/CD147 Double Nickase Plasmid (h) | sc-400589-NIC | 20 µg | $410.00 | |||
EMMPRIN/CD147 Double Nickase Plasmid (h2) | sc-400589-NIC-2 | 20 µg | $410.00 |
Basigin (BSG), also known as EMMPRIN/CD147, is a highly glycosylated immunoglobulin superfamily transmembrane protein that organizes cell–cell and cell–matrix interactions and modulates multiple signaling networks. It is best known for stimulating matrix metalloproteinase (MMP) production and facilitating extracellular matrix remodeling, with downstream effects on invasion, angiogenic responses, and inflammatory signaling. CD147 also functions as a chaperone for monocarboxylate transporters (e.g., MCT1/MCT4), linking lactate transport to metabolic reprogramming and hypoxia-adaptive pathways. Dysregulated BSG expression is associated with tumor progression, fibrosis, and pathogen host–cell interactions, making it a central node for studies of microenvironmental remodeling and metabolic coupling.
EMMPRIN/CD147 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BSG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BSG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BSG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BSG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.