emerin Antibody (A-5): sc-515522
- emerin Antibody (A-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-28 near the N-terminus of emerin of human origin
- recommended for detection of emerin of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for emerin Antibody (A-5) for WB applications. These reagents are now offered in bundles with emerin Antibody (A-5) (see ordering information below).
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Emerin Antibody (A-5) is a mouse monoclonal IgG1 antibody that detects emerin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Emerin is a crucial component of the nuclear lamina, playing a significant role in maintaining the structural integrity of the nuclear envelope and facilitating chromatin organization. Emerin′s localization to the nuclear membrane is vital for proper nuclear function, as emerin helps anchor the nuclear lamina to the inner nuclear membrane, thereby influencing gene expression and cellular signaling pathways. Mutations in the gene encoding emerin can lead to the X-linked recessive disorder Emery-Dreyfuss muscular dystrophy (EDMD), which manifests as progressive muscle weakness, contractures, and cardiac issues. Emerin′s absence disrupts the normal association between the nuclear membrane and the lamina, contributing to the pathophysiology of EDMD. Emerin′s specific localization to desmosomes and fasciae adherentes in cardiac tissue may explain the conduction defects observed in affected patients, highlighting emerin′s critical role in both muscle and cardiac health.
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emerin Antibody (A-5) References:
- Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein. | Ostlund, C., et al. 1999. J Cell Sci. 112 (Pt 11): 1709-19. PMID: 10318763
- BAF is required for emerin assembly into the reforming nuclear envelope. | Haraguchi, T., et al. 2001. J Cell Sci. 114: 4575-85. PMID: 11792822
- Association of emerin with nuclear and cytoplasmic actin is regulated in differentiating myoblasts. | Lattanzi, G., et al. 2003. Biochem Biophys Res Commun. 303: 764-70. PMID: 12670476
- Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. | Holaska, JM., et al. 2004. PLoS Biol. 2: E231. PMID: 15328537
- The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity. | Jacque, JM. and Stevenson, M. 2006. Nature. 441: 641-5. PMID: 16680152
- The Emery-Dreifuss muscular dystrophy associated-protein emerin is phosphorylated on serine 49 by protein kinase A. | Roberts, RC., et al. 2006. FEBS J. 273: 4562-75. PMID: 16972941
- Tyrosine phosphorylation of nuclear-membrane protein emerin by Src, Abl and other kinases. | Tifft, KE., et al. 2009. J Cell Sci. 122: 3780-90. PMID: 19789182
- Partial deficiency of emerin caused by a splice site mutation in EMD. | Yuan, J., et al. 2014. Intern Med. 53: 1563-8. PMID: 25030574
- Mutations in Emery-Dreifuss muscular dystrophy and their effects on emerin protein expression. | Manilal, S., et al. 1998. Hum Mol Genet. 7: 855-64. PMID: 9536090
- Colocalization of emerin and lamins in interphase nuclei and changes during mitosis. | Manilal, S., et al. 1998. Biochem Biophys Res Commun. 249: 643-7. PMID: 9731189
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
emerin Antibody (A-5) | sc-515522 | 200 µg/ml | $322.00 | |||
emerin Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-541063 | 200 µg Ab; 10 µg BP | $361.00 | |||
emerin Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-542558 | 200 µg Ab; 20 µg BP | $361.00 | |||
emerin (A-5) Neutralizing Peptide | sc-515522 P | 100 µg/0.5 ml | $69.00 |