Embigin Double Nickase Plasmid (h): sc-408321-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Embigin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Embigin Double Nickase Plasmid (h) and Embigin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EMB. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Embigin Double Nickase Plasmid (h) | sc-408321-NIC | 20 µg | $410.00 | |||
Embigin Double Nickase Plasmid (h2) | sc-408321-NIC-2 | 20 µg | $410.00 |
Human EMB encodes embigin, a type I transmembrane immunoglobulin superfamily glycoprotein that functions as an accessory molecule for monocarboxylate transporters and contributes to cell–cell and cell–matrix interactions. By modulating lactate and other monocarboxylate flux and participating in adhesion-linked signaling, embigin can influence metabolic homeostasis, membrane trafficking, and cellular migration programs. EMB expression has been examined in the context of tissue remodeling and dysregulated metabolism, making it relevant for studies of tumor-associated metabolic adaptation and microenvironmental interactions. Its surface localization and partnering behavior support mechanistic interrogation of transporter regulation and adhesion-dependent pathways.
Embigin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EMB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EMB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EMB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EMB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.