Elmo2 Antibody (C-12): sc-365739

Elmo2 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Elmo2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Elmo2 antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Elmo2 plays a crucial role in regulating cytoskeletal dynamics, particularly in processes such as cell motility and phagocytosis of apoptotic cells. Elmo2 interacts with Rho G in a GTP-dependent manner, forming a ternary complex with DOCK 180, which is essential for Rac 1 activation. This pathway is vital for integrin-mediated cell spreading and neurite outgrowth induced by nerve growth factor, highlighting Elmo2′s importance in cellular responses to environmental cues. Additionally, Elmo2′s interaction with other proteins, such as DOCK 180 and Rho G, underscores its role in orchestrating complex signaling pathways that govern cell behavior. Elmo2′s ability to influence these processes makes it a significant target for research in developmental biology and cancer metastasis, where cell motility is a key factor.

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Elmo2 Antibody (C-12) References:

1. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration.  |  Gumienny, TL., et al. 2001. Cell. 107: 27-41. PMID: 11595183
2. Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex.  |  Brugnera, E., et al. 2002. Nat Cell Biol. 4: 574-82. PMID: 12134158
3. DOCK2 regulates Rac activation and cytoskeletal reorganization through interaction with ELMO1.  |  Sanui, T., et al. 2003. Blood. 102: 2948-50. PMID: 12829596
4. RhoG activates Rac1 by direct interaction with the Dock180-binding protein Elmo.  |  Katoh, H. and Negishi, M. 2003. Nature. 424: 461-4. PMID: 12879077
5. PH domain of ELMO functions in trans to regulate Rac activation via Dock180.  |  Lu, M., et al. 2004. Nat Struct Mol Biol. 11: 756-62. PMID: 15247908
6. Phagocytosis of apoptotic cells is regulated by a UNC-73/TRIO-MIG-2/RhoG signaling module and armadillo repeats of CED-12/ELMO.  |  deBakker, CD., et al. 2004. Curr Biol. 14: 2208-16. PMID: 15620647
7. C-terminal SH3 domain of CrkII regulates the assembly and function of the DOCK180/ELMO Rac-GEF.  |  Akakura, S., et al. 2005. J Cell Physiol. 204: 344-51. PMID: 15700267
8. Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.  |  Yokoyama, N., et al. 2005. Biochemistry. 44: 8841-9. PMID: 15952790
9. The DOCK180/Elmo complex couples ARNO-mediated Arf6 activation to the downstream activation of Rac1.  |  Santy, LC., et al. 2005. Curr Biol. 15: 1749-54. PMID: 16213822
10. An ELMO2-RhoG-ILK network modulates microtubule dynamics.  |  Jackson, BC., et al. 2015. Mol Biol Cell. 26: 2712-25. PMID: 25995380
11. Elmo2 Is a Regulator of Insulin-dependent Glut4 Membrane Translocation.  |  Sun, Y., et al. 2016. J Biol Chem. 291: 16150-61. PMID: 27226625
12. ELMO2 association with Gαi2 regulates pancreatic cancer cell chemotaxis and metastasis.  |  Wang, Y., et al. 2020. PeerJ. 8: e8910. PMID: 32292657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Elmo2 Antibody (C-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Elmo2 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Elmo2 Antibody (C-12) Specifications

Alternate Product Name: Elmo2

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-551

Epitope Target: Elmo2

Epitope Species: mouse

Product Reactivity/Detection: Elmo1 and Elmo2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Elmo2 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Elmo1 antibody is Elmo1 Antibody (B-7): sc-271519.