Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
elastin Double Nickase Plasmid (h): sc-400640-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- elastin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- elastin Double Nickase Plasmid (h) and elastin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ELN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: elastin Antibody (E-11): sc-166543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
elastin Double Nickase Plasmid (h) | sc-400640-NIC | 20 µg | $410.00 | |||
elastin Double Nickase Plasmid (h2) | sc-400640-NIC-2 | 20 µg | $410.00 |
ELN encodes elastin, a core extracellular matrix protein that assembles into elastic fibers to provide recoil and resilience in arteries, lung, and skin. Elastin monomers are secreted and crosslinked during elastogenesis through interactions with microfibrillar scaffolds and lysyl oxidase–mediated maturation, supporting tissue biomechanics and mechanotransduction. Perturbations in elastin abundance or organization influence vascular remodeling and extracellular matrix homeostasis, intersecting with pathways that regulate smooth muscle cell phenotype and connective tissue integrity. ELN dysfunction and elastic fiber fragmentation are associated with heritable and acquired disorders of vascular and connective tissues, making it a key locus for studying matrix biology.
elastin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ELN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ELN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ELN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ELN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.