eIF4H Antibody (C-6): sc-515265
- eIF4H Antibody (C-6) is a mouse monoclonal IgG1 κ eIF4H antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 51-201 mapping within an internal region of eIF4H of human origin
- eIF4H Antibody (C-6) is recommended for detection of eIF4H of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eIF4H Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eIF4H Antibody (C-6) for WB applications. These reagents are now offered in bundles with eIF4H Antibody (C-6) (see ordering information below).
eIF4H Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF4H of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF4H antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4H, also known as eukaryotic translation initiation factor 4H, plays a crucial role in regulating protein synthesis by enhancing eIF4A activity within the translation initiation complex, thereby facilitating mRNA secondary structure unwinding and promoting ribosome assembly. This function is vital for efficient translation of mRNA into proteins, essential for cellular function and growth. eIF4H is predominantly localized to the perinuclear region of the cytoplasm and exists in two isoforms, with the short isoform primarily expressed in liver and kidney, while both isoforms are found in lung, pancreas, testis, and spleen. eIF4H′s importance is underscored by its association with Williams-Beuren syndrome (WBS), a genetic disorder leading to various developmental issues, including cardiovascular and musculoskeletal abnormalities, highlighting the critical role of proper protein synthesis in human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF4H Antibody (C-6) References:
- The Chironomus tentans translation initiation factor eIF4H is present in the nucleus but does not bind to mRNA until the mRNA reaches the cytoplasmic perinuclear region. | Bjork, P., et al. 2003. J Cell Sci. 116: 4521-32. PMID: 14576346
- Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H. | Doepker, RC., et al. 2004. J Virol. 78: 4684-99. PMID: 15078951
- Interaction between the NH2-terminal domain of eIF4A and the central domain of eIF4G modulates RNA-stimulated ATPase activity. | Korneeva, NL., et al. 2005. J Biol Chem. 280: 1872-81. PMID: 15528191
- mRNA decay during herpes simplex virus (HSV) infections: protein-protein interactions involving the HSV virion host shutoff protein and translation factors eIF4H and eIF4A. | Feng, P., et al. 2005. J Virol. 79: 9651-64. PMID: 16014927
- Stimulation of mammalian translation initiation factor eIF4A activity by a small molecule inhibitor of eukaryotic translation. | Bordeleau, ME., et al. 2005. Proc Natl Acad Sci U S A. 102: 10460-5. PMID: 16030146
- Cap-dependent eukaryotic initiation factor-mRNA interactions probed by cross-linking. | Lindqvist, L., et al. 2008. RNA. 14: 960-9. PMID: 18367715
- Small interfering RNAs that deplete the cellular translation factor eIF4H impede mRNA degradation by the virion host shutoff protein of herpes simplex virus. | Sarma, N., et al. 2008. J Virol. 82: 6600-9. PMID: 18448541
- Identification of genes from a 500-kb region at 7q11.23 that is commonly deleted in Williams syndrome patients. | Osborne, LR., et al. 1996. Genomics. 36: 328-36. PMID: 8812460
- Purification and characterization of a new eukaryotic protein translation factor. Eukaryotic initiation factor 4H. | Richter-Cook, NJ., et al. 1998. J Biol Chem. 273: 7579-87. PMID: 9516461
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4H Antibody (C-6) | sc-515265 | 200 µg/ml | $316.00 | |||
eIF4H Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-528012 | 200 µg Ab; 10 µg BP | $354.00 | |||
eIF4H Antibody (C-6): m-IgG1 BP-HRP Bundle | sc-533385 | 200 µg Ab; 20 µg BP | $354.00 | |||
eIF4H Antibody (C-6) AC | sc-515265 AC | 500 µg/ml, 25% agarose | $416.00 | |||
eIF4H Antibody (C-6) HRP | sc-515265 HRP | 200 µg/ml | $316.00 | |||
eIF4H Antibody (C-6) FITC | sc-515265 FITC | 200 µg/ml | $330.00 | |||
eIF4H Antibody (C-6) PE | sc-515265 PE | 200 µg/ml | $343.00 | |||
eIF4H Antibody (C-6) Alexa Fluor® 488 | sc-515265 AF488 | 200 µg/ml | $357.00 | |||
eIF4H Antibody (C-6) Alexa Fluor® 546 | sc-515265 AF546 | 200 µg/ml | $357.00 | |||
eIF4H Antibody (C-6) Alexa Fluor® 594 | sc-515265 AF594 | 200 µg/ml | $357.00 | |||
eIF4H Antibody (C-6) Alexa Fluor® 647 | sc-515265 AF647 | 200 µg/ml | $357.00 | |||
eIF4H Antibody (C-6) Alexa Fluor® 680 | sc-515265 AF680 | 200 µg/ml | $357.00 | |||
eIF4H Antibody (C-6) Alexa Fluor® 790 | sc-515265 AF790 | 200 µg/ml | $357.00 |