eIF4GII Antibody (R16S): sc-100732

eIF4GII Antibody (R16S) is a mouse monoclonal IgG2b kappa light chain antibody that detects eIF4GII protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eIF4GII antibody (R16S) is available as a non-conjugated monoclonal isotype antibody. eIF4GII protein, also known as eIF4G3, plays a crucial role in the initiation of translation in eukaryotic cells, which is essential for protein synthesis and cellular function. eIF4GII (R16S) monoclonal antibody is a key component of the eIF4F complex, which binds to the 5′ cap of mRNA and facilitates the recruitment of the ribosomal preinitiation complex, thereby promoting the assembly of the ribosome at the start codon of mRNA. eIF4GII protein is located in the cytoplasm, where eIF4GII interacts with other translation initiation factors, including eIF4E and eIF4A, to unwind mRNA secondary structures and ensure efficient translation. eIF4GII protein is 1,585 amino acids long and shares 46% homology with eIF4GI, highlighting functional importance in the translation process. Understanding eIF4GII′s role in translation initiation is vital, as dysregulation of this process can lead to various diseases, including cancer.

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eIF4GII Antibody (R16S) References:

1. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation.  |  Gingras, AC., et al. 1999. Annu Rev Biochem. 68: 913-63. PMID: 10872469
2. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo.  |  Asano, K., et al. 2000. Genes Dev. 14: 2534-46. PMID: 11018020
3. Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F.  |  Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
4. Initiation of protein synthesis in eukaryotic cells.  |  Pain, VM. 1996. Eur J Biochem. 236: 747-71. PMID: 8665893
5. A novel functional human eukaryotic translation initiation factor 4G.  |  Gradi, A., et al. 1998. Mol Cell Biol. 18: 334-42. PMID: 9418880
6. A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation.  |  Imataka, H., et al. 1998. EMBO J. 17: 7480-9. PMID: 9857202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4GII Antibody (R16S) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4GII Antibody (R16S) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4GII Antibody (R16S) Specifications

Alternate Product Name: EIF4G3

Product Clone Name: R16S

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-516

Epitope Target: eIF4GII

Epitope Species: human

Product Reactivity/Detection: eIF4GII

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for eIF4GII Antibody (R16S): N/A