Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4AIII Antibody (B-2): sc-365549. Western blot analysis of eIF4AIII expression in MCF7 (A) and HeLa (B) nuclear extracts and NIH/3T3 whole cell lysate (C). Detection reagent used: m-IgGκ BP-HRP: sc-516102.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4AIII Double Nickase Plasmid (m): sc-431393-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4AIII Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF4AIII Double Nickase Plasmid (m) and eIF4AIII Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Eif4a3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4AIII Antibody (B-2): sc-365549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4AIII Double Nickase Plasmid (m) | sc-431393-NIC | 20 µg | $410.00 | |||
eIF4AIII Double Nickase Plasmid (m2) | sc-431393-NIC-2 | 20 µg | $410.00 |