eIF2A Antibody (3A7A8): sc-517214

eIF2A Antibody (3A7A8) is a mouse monoclonal IgG1 antibody that detects eIF2A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). eIF2A (3A7A8) antibody is available as a non-conjugated format. eIF2A protein, also known as CDA02, MSTP004, or MSTP089, is a 585 amino acid protein characterized by three WD repeats, which are crucial for translation initiation process. eIF2A protein is ubiquitously expressed, with highest levels found in heart, brain, pancreas, and placenta, highlighting essential role in cellular metabolism and protein synthesis. eIF2A functions as a translation initiation factor by binding to methionyl-tRNA and directing it to 40S ribosomal subunits, a critical step in early stages of protein synthesis. Unlike eIF2 complex, which operates in a GTP-dependent manner, eIF2A facilitates this process in a codon-dependent manner, underscoring unique regulatory capabilities. Additionally, eIF2A may play a role in negative regulation of specific proteins, suggesting potential function as a transcriptional repressor. eIF2A exists in two isoforms due to alternative splicing, which may contribute to diverse functional roles in different cellular contexts.

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eIF2A Antibody (3A7A8) References:

1. Characterization of mammalian eIF2A and identification of the yeast homolog.  |  Zoll, WL., et al. 2002. J Biol Chem. 277: 37079-87. PMID: 12133843
2. Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A.  |  Komar, AA., et al. 2005. J Biol Chem. 280: 15601-11. PMID: 15718232
3. Fibronectin controls cap-dependent translation through beta1 integrin and eukaryotic initiation factors 4 and 2 coordinated pathways.  |  Gorrini, C., et al. 2005. Proc Natl Acad Sci U S A. 102: 9200-5. PMID: 15961545
4. Mechanism and regulation of eukaryotic protein synthesis.  |  Merrick, WC. 1992. Microbiol Rev. 56: 291-315. PMID: 1620067
5. Double-stranded RNA-dependent protein kinase phosphorylation of the alpha-subunit of eukaryotic translation initiation factor 2 mediates apoptosis.  |  Scheuner, D., et al. 2006. J Biol Chem. 281: 21458-21468. PMID: 16717090
6. Inhibition of ribosome recruitment induces stress granule formation independently of eukaryotic initiation factor 2alpha phosphorylation.  |  Mazroui, R., et al. 2006. Mol Biol Cell. 17: 4212-9. PMID: 16870703
7. Phosphorylation of eIF2 directs ATF5 translational control in response to diverse stress conditions.  |  Zhou, D., et al. 2008. J Biol Chem. 283: 7064-73. PMID: 18195013
8. MEK signaling is required for phosphorylation of eIF2alpha following amino acid limitation of HepG2 human hepatoma cells.  |  Thiaville, MM., et al. 2008. J Biol Chem. 283: 10848-57. PMID: 18287093

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF2A Antibody (3A7A8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF2A Antibody (3A7A8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF2A Antibody (3A7A8) Specifications

Alternate Product Name: eIF2A

Product Clone Name: 3A7A8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 448-576

Epitope Target: eIF2A

Epitope Species: human

Product Reactivity/Detection: eIF2A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: monkey

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for eIF2A Antibody (3A7A8): N/A