EGFL6 Double Nickase Plasmid (h): sc-411850-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EGFL6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EGFL6 Double Nickase Plasmid (h) and EGFL6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EGFL6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EGFL6 Double Nickase Plasmid (h) | sc-411850-NIC | 20 µg | $410.00 | |||
EGFL6 Double Nickase Plasmid (h2) | sc-411850-NIC-2 | 20 µg | $410.00 |
EGFL6 encodes epidermal growth factor-like protein 6, a secreted extracellular matrix–associated factor enriched in vascular and developmental contexts. It contributes to cell–matrix interactions, endothelial and stromal cell behavior, and regulation of migration and adhesion through signaling networks that intersect with integrin-mediated pathways and growth factor–responsive programs. EGFL6 expression has been associated with tissue remodeling and angiogenic processes that are frequently altered in tumor microenvironments and other pathologies involving aberrant vascularization. As a result, EGFL6 is commonly studied for its roles in extracellular signaling, matrix organization, and modulation of proliferative and motility phenotypes in human cell models.
EGFL6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EGFL6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EGFL6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EGFL6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EGFL6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.