Date published: 2025-9-29

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EDC hydrochloride (CAS 25952-53-8)

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Alternate Names:
EDC hydrochloride is also known as (3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride.
Application:
EDC hydrochloride is a water soluble carboxyl activating carbodiimide reagent, used for amide bond formation.
CAS Number:
25952-53-8
Purity:
≥98%
Molecular Weight:
191.70
Molecular Formula:
C8H17N3•HCl
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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EDC hydrochloride is a coupling reagent. Classified as a carbodiimide, it is carboxyl activating and water soluble. EDC hydrochloride is used for amide bond formation in oligonucleotides and tetraazamacrocycles. Since it is water soluble, it is a viable component to use in the preparation of chloroanthraquinone hydrazides. Additionally, EDC hdyrochloride may be used to activate phosphate groups, synthesize peptides, crosslink proteins to nucleic acids, and prepare immunoconjugates.


EDC hydrochloride (CAS 25952-53-8) References

  1. Convenient synthesis of bifunctional tetraaza macrocycles.  |  McMurry, TJ., et al. 1992. Bioconjug Chem. 3: 108-17. PMID: 1515464
  2. New analogues of acyclovir--synthesis and biological activity.  |  Stankova, I., et al. 2010. Z Naturforsch C J Biosci. 65: 29-33. PMID: 20355317
  3. Improved sensitivity mass spectrometric detection of eicosanoids by charge reversal derivatization.  |  Bollinger, JG., et al. 2010. Anal Chem. 82: 6790-6. PMID: 20704368
  4. Development of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese.  |  Pimenta-Martins, MG., et al. 2012. J Microbiol Methods. 91: 138-43. PMID: 22710410
  5. Biocompatible glycol chitosan-coated gold nanoparticles for tumor-targeting CT imaging.  |  Sun, IC., et al. 2014. Pharm Res. 31: 1418-25. PMID: 23934255
  6. Interactions of fish gelatin and chitosan in uncrosslinked and crosslinked with EDC films: FT-IR study.  |  Staroszczyk, H., et al. 2014. Spectrochim Acta A Mol Biomol Spectrosc. 117: 707-12. PMID: 24140742
  7. Fabrication of photo-crosslinked chitosan- gelatin scaffold in sodium alginate hydrogel for chondrocyte culture.  |  Zhao, P., et al. 2014. Biomed Mater Eng. 24: 633-41. PMID: 24211948
  8. Synthesis and characterization of pH tolerant and mucoadhesive (thiol-polyethylene glycol) chitosan graft polymer for drug delivery.  |  Hauptstein, S., et al. 2014. J Pharm Sci. 103: 594-601. PMID: 24382680
  9. Experimental and theoretical studies on Gallic acid assisted EDC/NHS initiated crosslinked collagen scaffolds.  |  Krishnamoorthy, G., et al. 2014. Mater Sci Eng C Mater Biol Appl. 43: 164-71. PMID: 25175201
  10. Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering.  |  Li, W., et al. 2014. J Biomater Sci Polym Ed. 25: 1962-72. PMID: 25299624
  11. Towards Elucidating the Effects of Purified MWCNTs on Human Lung Epithelial cells.  |  Dong, C., et al. 2014. Environ Sci Nano. 1: 95-603. PMID: 25485116
  12. Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 2: Mass spectrometric methods.  |  Reusch, D., et al. 2015. MAbs. 7: 732-42. PMID: 25996192
  13. Carbon nanodots as molecular scaffolds for development of antimicrobial agents.  |  Ngu-Schwemlein, M., et al. 2016. Bioorg Med Chem Lett. 26: 1745-9. PMID: 26923697
  14. Functional Nanogel from Natural Substances for Delivery of Doxorubicin.  |  Kamenova, K., et al. 2022. Polymers (Basel). 14: PMID: 36080768

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

EDC hydrochloride, 1 g

sc-219152A
1 g
$28.00

EDC hydrochloride, 10 g

sc-219152
10 g
$105.00

EDC hydrochloride, 250 g

sc-219152B
250 g
$229.00

EDC hydrochloride, 1 kg

sc-219152C
1 kg
$550.00

EDC hydrochloride, 5 kg

sc-219152D
5 kg
$2625.00

Will the chemical be affected if the storage temperature went down to -30 C

Asked by: Lucas
Thank you for your question. -30 C will not affect the chemical.
Answered by: BlakeJ
Date published: 2024-07-09
  • y_2025, m_9, d_24, h_6CST
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Rated 5 out of 5 by from works well for chemical couplingI have used this EDC exclusively for carbodiimide coupling reactions for the past year. This reagent is of very high quality and is cheaper than other sources.
Date published: 2017-02-14
Rated 5 out of 5 by from Great reagent for peptide couplingGreat reagent for peptide coupling, easier workup than DCC.
Date published: 2015-10-13
Rated 5 out of 5 by from FrostFrost, L. et al. (PubMed 26114780) using combined approaches one of which was EDC hydrochloride, a water soluble carboxyl activating reagent for amide bond formation, chemical cross-linking, to discover that HMGA2 is capable of self-associating into homodimers in aqueous buffer solutions. -SCBT Publication Review
Date published: 2015-05-03
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EDC hydrochloride is rated 5.0 out of 5 by 3.
  • y_2025, m_9, d_24, h_6
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