ECA39 Antibody (1F8): sc-517185

ECA39 Antibody (1F8) is a mouse monoclonal IgG2a kappa light chain antibody that detects the ECA39 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ECA39 antibody (1F8) is available as the non-conjugated format. ECA39, also known as branched-chain-amino-acid aminotransferase 1 (BCAT1), plays a crucial role in the metabolism of branched-chain amino acids, specifically valine, leucine, and isoleucine, which are essential for protein synthesis and energy production. ECA39 is primarily localized in the cytoplasm, where ECA39 forms a homodimer, and is particularly important during embryogenesis, as ECA39 is expressed in the brain and kidney. Notably, ECA39 is overexpressed in tumors induced by c-Myc, highlighting ECA39′s potential role in cancer biology. ECA39′s interaction with other proteins, such as BCAT2, which is a mitochondrial isoform of the enzyme, further emphasizes ECA39′s significance in metabolic pathways. BCAT2, also known as placental protein 18, exists in two isoforms due to alternative splicing, with BCAT2A localized in the mitochondrion and BCAT2B in the cytoplasm, suggesting a complex regulatory mechanism in amino acid metabolism. ECA39′s ability to interact with these isoforms underscores ECA39′s importance in maintaining cellular homeostasis and potential implications in metabolic disorders and cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ECA39 Antibody (1F8) References:

1. Involvement of branched-chain amino acid aminotransferase (Bcat1/Eca39) in apoptosis.  |  Eden, A. and Benvenisty, N. 1999. FEBS Lett. 457: 255-61. PMID: 10471790
2. Lrmp and Bcat1 are candidates for the type I diabetes susceptibility locus Idd6.  |  Grimm, CH., et al. 2003. Autoimmunity. 36: 241-6. PMID: 14563018
3. ECA39 is a novel distant metastasis-related biomarker in colorectal cancer.  |  Yoshikawa, R., et al. 2006. World J Gastroenterol. 12: 5884-9. PMID: 17007058
4. Functional evidence for a nasopharyngeal carcinoma-related gene BCAT1 located at 12p12.  |  Zhou, W., et al. 2007. Oncol Res. 16: 405-13. PMID: 18074675
5. Regulatory control of human cytosolic branched-chain aminotransferase by oxidation and S-glutathionylation and its interactions with redox sensitive neuronal proteins.  |  Conway, ME., et al. 2008. Biochemistry. 47: 5465-79. PMID: 18419134
6. ECA39, a conserved gene regulated by c-Myc in mice, is involved in G1/S cell cycle regulation in yeast.  |  Schuldiner, O., et al. 1996. Proc Natl Acad Sci U S A. 93: 7143-8. PMID: 8692959
7. Characterization of murine BCAT genes: Bcat1, a c-Myc target, and its homolog, Bcat2.  |  Ben-Yosef, T., et al. 1998. Mamm Genome. 9: 595-7. PMID: 9657861