Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EBP1 CRISPR/Cas9 KO Plasmid (h): sc-402177
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EBP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EBP1 Antibody (C-11): sc-393114
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EBP1 CRISPR/Cas9 KO Plasmid (h) | sc-402177 | 20 µg | $397.00 |
Overview
PA2G4 encodes EBP1, an RNA- and protein-binding factor that localizes to both cytoplasm and nucleus and participates in the coordination of gene expression at transcriptional and translational levels. EBP1 has been linked to signaling downstream of receptor tyrosine kinases and to the regulation of cell-cycle progression, proliferation, and differentiation through modulation of mRNA stability, ribosome-associated processes, and transcriptional programs. By influencing growth control pathways and stress-responsive gene expression networks, PA2G4 is frequently studied in the context of oncogenic signaling and other diseases characterized by dysregulated proliferation. Its multifaceted roles make it a useful node for dissecting how growth factor cues are integrated with RNA metabolism and chromatin-associated regulation in human cells.
EBP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PA2G4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PA2G4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PA2G4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EBP1 protein expression.
This CRISPR knockout system enables efficient generation of PA2G4-deficient cell models for investigation of EBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PA2G4 exon(s) critical for EBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PA2G4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EBP1 CRISPR/Cas9 KO Plasmid (h) and EBP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PA2G4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EBP1 HDR Plasmid (h) and EBP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PA2G4 homology arms to support homology-directed repair at defined PA2G4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.