EBF3 Double Nickase Plasmid (h): sc-402181-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EBF3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EBF3 Double Nickase Plasmid (h) and EBF3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EBF3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EBF3 Antibody (N7Q): sc-81999
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EBF3 Double Nickase Plasmid (h) | sc-402181-NIC | 20 µg | $410.00 | |||
EBF3 Double Nickase Plasmid (h2) | sc-402181-NIC-2 | 20 µg | $410.00 |
EBF3 encodes an early B-cell factor family transcription factor that binds DNA through a conserved helix–loop–helix motif to regulate gene programs controlling neuronal differentiation, cell-cycle progression, and lineage specification. In human cells, EBF3 integrates with transcriptional networks that shape chromatin accessibility and coordinate developmental timing across neural and mesenchymal contexts. Perturbation of EBF3 has been linked to neurodevelopmental disorders, including syndromic presentations with intellectual disability and ataxia, consistent with its role in neuronal maturation and circuit formation. Because EBF3 influences broad transcriptional cascades, it is frequently studied in models of developmental gene regulation, cellular identity, and stress-responsive transcription.
EBF3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EBF3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EBF3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EBF3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EBF3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.