EBF3 Antibody (N7Q): sc-81999

EBF3 Antibody (N7Q) is a mouse monoclonal IgG1 kappa light chain antibody that detects EBF3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-EBF3 antibody (N7Q) is available as the non-conjugated form. EBF3 plays a crucial role in B lymphocyte maturation, a process essential for adaptive immune response. As a transcription factor, EBF3 regulates gene expression necessary for B cell development, including components of B cell receptor and B lymphoid kinase, which are vital for initiating immunoglobulin light chain gene recombination and signaling pathways. EBF3 is predominantly localized in the nucleus and can form homodimers or heterodimers with other family members, enhancing regulatory capabilities. EBF3′s ability to bind specific DNA sequences, particularly the core sequence CCCNNGGG, through a zinc-coordination motif in DNA-recognition domain underscores importance in transcriptional activation. Additionally, EBF3 exists in two isoforms due to alternative splicing, which may contribute to functional diversity in various cellular contexts.

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EBF3 Antibody (N7Q) References:

1. Early B cell factor is an activator of the B lymphoid kinase promoter in early B cell development.  |  Akerblad, P. and Sigvardsson, M. 1999. J Immunol. 163: 5453-61. PMID: 10553071
2. Overlapping expression of early B-cell factor and basic helix-loop-helix proteins as a mechanism to dictate B-lineage-specific activity of the lambda5 promoter.  |  Sigvardsson, M. 2000. Mol Cell Biol. 20: 3640-54. PMID: 10779354
3. EBF3 reactivation by inhibiting the EGR1/EZH2/HDAC9 complex promotes metastasis via transcriptionally enhancing vimentin in nasopharyngeal carcinoma.  |  Ding, S., et al. 2022. Cancer Lett. 527: 49-65. PMID: 34906623
4. Duplication/triplication mosaicism of EBF3 and expansion of the EBF3 neurodevelopmental disorder phenotype.  |  Ignatius, E., et al. 2022. Eur J Paediatr Neurol. 37: 1-7. PMID: 34999443
5. Neurologic, Neuropsychologic, and Neuroradiologic Features of EBF3-Related Syndrome.  |  Ciaccio, C., et al. 2023. Neurol Genet. 9: e200049. PMID: 37090941
6. Circadian clock protein Bmal1 accelerates acute myeloid leukemia by inhibiting ferroptosis through the EBF3/ALOX15 axis.  |  Wang, D., et al. 2023. Cancer Sci. 114: 3446-3460. PMID: 37271497
7. Ebf3+ niche-derived CXCL12 is required for the localization and maintenance of hematopoietic stem cells.  |  Nakatani, T., et al. 2023. Nat Commun. 14: 6402. PMID: 37880234
8. Failure of B-cell differentiation in mice lacking the transcription factor EBF.  |  Lin, H. and Grosschedl, R. 1995. Nature. 376: 263-7. PMID: 7542362
9. EBF contains a novel zinc coordination motif and multiple dimerization and transcriptional activation domains.  |  Hagman, J., et al. 1995. EMBO J. 14: 2907-16. PMID: 7796816
10. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast.  |  Wang, MM. and Reed, RR. 1993. Nature. 364: 121-6. PMID: 8321284
11. EBF and E47 collaborate to induce expression of the endogenous immunoglobulin surrogate light chain genes.  |  Sigvardsson, M., et al. 1997. Immunity. 7: 25-36. PMID: 9252117
12. The B29 (immunoglobulin beta-chain) gene is a genetic target for early B-cell factor.  |  Akerblad, P., et al. 1999. Mol Cell Biol. 19: 392-401. PMID: 9858563

The preferred EBF antibody is EBF Antibody (C-8): sc-137065.