EAR2 Lentiviral Activation Particles (m): sc-420216-LAC
- Target species: mouse
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- EAR2 Lentiviral Activation Particles (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- EAR2 Lentiviral Activation Particles (m) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by EAR2 Lentiviral Activation Plasmid (m) and EAR2 Lentiviral Activation Plasmid (m2) target distinct regulatory regions of the Nr2f6 promoter. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EAR2 Lentiviral Activation Particles (m) | sc-420216-LAC | 200 µl | $455.00 |
Nr2f6 encodes EAR2 (NR2F6), an orphan nuclear receptor that functions as a DNA-binding transcriptional regulator coordinating lineage programs and stimulus-dependent gene expression. In mouse cells, EAR2 integrates into nuclear receptor networks to modulate transcription at hormone response elements and related cis-regulatory motifs, influencing immune signaling, inflammatory gene programs, and cellular differentiation states. Through these roles, NR2F6/EAR2 has been studied in contexts including T cell functional polarization, innate immune responses, and tumor–immune interactions, where altered transcriptional control can shift cytokine pathways and cellular fitness. As a regulator of transcriptional homeostasis, EAR2 is relevant for mechanistic studies of immune regulation, epigenetic control, and stress-responsive gene networks.
EAR2 Lentiviral Activation Particles (m) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient Nr2f6 upregulation across a broader range of human cell types.
EAR2 Lentiviral Activation Particles (m) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the Nr2f6 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous EAR2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native Nr2f6 genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.