Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EAR2 Double Nickase Plasmid (h): sc-404509-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EAR2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EAR2 Double Nickase Plasmid (h) and EAR2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR2F6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EAR2 Double Nickase Plasmid (h) | sc-404509-NIC | 20 µg | $410.00 | |||
EAR2 Double Nickase Plasmid (h2) | sc-404509-NIC-2 | 20 µg | $410.00 |
NR2F6 (EAR2) encodes an orphan nuclear receptor that functions as a sequence-specific transcription factor linking ligand-independent nuclear receptor signaling to chromatin-dependent gene regulation. EAR2 modulates transcriptional programs involved in immune homeostasis, inflammatory signaling, and cell fate decisions, intersecting with pathways such as cytokine-driven JAK/STAT and NF-κB-regulated gene networks through promoter/enhancer occupancy and coregulator recruitment. In human cells, altered NR2F6 activity has been associated with dysregulated T cell responses and broader immune-oncology and inflammatory disease biology, making it relevant for studying context-dependent transcriptional repression/activation. NR2F6 is also used as a node for dissecting nuclear receptor crosstalk and identifying regulatory elements that control lineage-specific gene expression.
EAR2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR2F6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR2F6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR2F6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR2F6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.