E-Syt1 Antibody (C-1): sc-514488
- E-Syt1 Antibody (C-1) is a mouse monoclonal IgG2a κ E-Syt1 antibody provided at 200 µg/ml
- raised against amino acids 42-130 mapping near the N-terminus of E-Syt1 of human origin
- E-Syt1 Antibody (C-1) is recommended for detection of E-Syt1 of human origin by WB, IP, IF and ELISA
- Anti-E-Syt1 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for E-Syt1 Antibody (C-1) for WB applications. This reagent is now offered in a bundle with E-Syt1 Antibody (C-1) (see ordering information below).
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E-Syt1 Antibody (C-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects E-Syt1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E-Syt1 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E-Syt1, also known as extended synaptotagmin-like protein 1, plays a significant role in regulating membrane fusion events, particularly in neurotransmitter release. E-Syt1 is primarily located in the endoplasmic reticulum and plasma membrane, where E-Syt1 forms junctions that facilitate communication between these two critical cellular compartments. E-Syt1 presence at these junctions maintains calcium homeostasis and lipid transfer, which are essential for proper cellular signaling and function. E-Syt1 is a 1,104 amino acid protein that exists in two alternatively spliced isoforms and is encoded by a gene located on human chromosome 12q13.2. Members of the Extended Synaptotagmins family, including E-Syt1, E-Syt2, and E-Syt3, are highly conserved across species and form heteromeric complexes, underscoring their importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E-Syt1 Antibody (C-1) References:
- E-Syts, a family of membranous Ca2+-sensor proteins with multiple C2 domains. | Min, SW., et al. 2007. Proc Natl Acad Sci U S A. 104: 3823-8. PMID: 17360437
- Evolutionary genomics of plant genes encoding N-terminal-TM-C2 domain proteins and the similar FAM62 genes and synaptotagmin genes of metazoans. | Craxton, M. 2007. BMC Genomics. 8: 259. PMID: 17672888
- A manual collection of Syt, Esyt, Rph3a, Rph3al, Doc2, and Dblc2 genes from 46 metazoan genomes--an open access resource for neuroscience and evolutionary biology. | Craxton, M. 2010. BMC Genomics. 11: 37. PMID: 20078875
- PI(4,5)P(2)-dependent and Ca(2+)-regulated ER-PM interactions mediated by the extended synaptotagmins. | Giordano, F., et al. 2013. Cell. 153: 1494-509. PMID: 23791178
- Proteomic and genomic evidence implicates the postsynaptic density in schizophrenia. | Föcking, M., et al. 2015. Mol Psychiatry. 20: 424-32. PMID: 25048004
- Loss of Extended Synaptotagmins ESyt2 and ESyt3 does not affect mouse development or viability, but in vitro cell migration and survival under stress are affected. | Herdman, C., et al. 2014. Cell Cycle. 13: 2616-25. PMID: 25486202
- Extended Synaptotagmin Interaction with the Fibroblast Growth Factor Receptor Depends on Receptor Conformation, Not Catalytic Activity. | Tremblay, MG., et al. 2015. J Biol Chem. 290: 16142-56. PMID: 25922075
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E-Syt1 Antibody (C-1) | sc-514488 | 200 µg/ml | $316.00 | |||
E-Syt1 Antibody (C-1): m-IgG2a BP-HRP Bundle | sc-545977 | 200 µg Ab; 10 µg BP | $354.00 | |||
E-Syt1 Antibody (C-1) AC | sc-514488 AC | 500 µg/ml, 25% agarose | $416.00 | |||
E-Syt1 Antibody (C-1) HRP | sc-514488 HRP | 200 µg/ml | $316.00 | |||
E-Syt1 Antibody (C-1) FITC | sc-514488 FITC | 200 µg/ml | $330.00 | |||
E-Syt1 Antibody (C-1) PE | sc-514488 PE | 200 µg/ml | $343.00 | |||
E-Syt1 Antibody (C-1) Alexa Fluor® 488 | sc-514488 AF488 | 200 µg/ml | $357.00 | |||
E-Syt1 Antibody (C-1) Alexa Fluor® 546 | sc-514488 AF546 | 200 µg/ml | $357.00 | |||
E-Syt1 Antibody (C-1) Alexa Fluor® 594 | sc-514488 AF594 | 200 µg/ml | $357.00 | |||
E-Syt1 Antibody (C-1) Alexa Fluor® 647 | sc-514488 AF647 | 200 µg/ml | $357.00 | |||
E-Syt1 Antibody (C-1) Alexa Fluor® 680 | sc-514488 AF680 | 200 µg/ml | $357.00 | |||
E-Syt1 Antibody (C-1) Alexa Fluor® 790 | sc-514488 AF790 | 200 µg/ml | $357.00 |