E-Selectin/CD62E/SELE (h): 293T Lysate: sc-112627

The E-Selectin (h): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line noted for its high transfection efficiency and widespread use in molecular and cellular biology research. This lysate contains E-Selectin, an endothelial cell adhesion molecule that plays a critical role in the inflammatory response by mediating the adhesion and rolling of leukocytes on the vascular endothelium. E-Selectin is upregulated in response to pro-inflammatory cytokines like IL-1 and TNF-α. The interaction of E-Selectin with its ligands on leukocytes initiates a cascade of signaling events, leading to the firm adhesion and extravasation of immune cells into tissues. In research, the E-Selectin (h): 293T Lysate is used to study the molecular mechanisms underlying leukocyte-endothelial interactions. Researchers utilize this lysate to investigate the binding specificity and kinetics of E-Selectin with various glycoprotein ligands, analyze post-translational modifications, and explain the signaling pathways triggered by E-Selectin engagement. It serves as a valuable tool in in vitro assays to screen for potential modulators of E-Selectin activity, helping to understand the regulation of immune cell trafficking and the broader implications of E-Selectin in inflammation and immune responses. By leveraging this lysate, scientists gain deeper insights into the cellular and molecular processes governing vascular inflammation and immune cell migration.